GeoChip: a comprehensive microarray for investigating biogeochemical, ecological and environmental processes.

GeoChip: a comprehensive microarray for investigating biogeochemical, ecological and environmental processes. Research Abstract Details 

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GeoChip: a comprehensive microarray for investigating biogeochemical, ecological and environmental processes. Abstract Text:

Zhili He,Terry J Gentry,Christopher W Schadt,Liyou Wu,Jost Liebich,Song C Chong,Zhijian Huang,Weimin Wu,Baohua Gu,Phil Jardine,Craig Criddle,Jizhong Zhou,Zhili He,Terry J Gentry,Christopher W Schadt,Liyou Wu,Jost Liebich,Song C Chong,Zhijian Huang,Weimin Wu,Baohua Gu,Phil Jardine,Craig Criddle,Jizhong Zhou,Zhili He,Terry J Gentry,Christopher W Schadt,Liyou Wu,Jost Liebich,Song C Chong,Zhijian Huang,Weimin Wu,Baohua Gu,Phil Jardine,Craig Criddle,Jizhong Zhou,

Owing to their vast diversity and as-yet uncultivated status, detection, characterization and quantification of microorganisms in natural settings are very challenging, and linking microbial diversity to ecosystem processes and functions is even more difficult. Microarray-based genomic technology for detecting functional genes and processes has a great promise of overcoming such obstacles. Here, a novel comprehensive microarray, termed GeoChip, has been developed, containing 24 243 oligonucleotide (50 mer) probes and covering >10 000 genes in >150 functional groups involved in nitrogen, carbon, sulfur and phosphorus cycling, metal reduction and resistance, and organic contaminant degradation. The developed GeoChip was successfully used for tracking the dynamics of metal-reducing bacteria and associated communities for an in situ bioremediation study. This is the first comprehensive microarray currently available for studying biogeochemical processes and functional activities of microbial communities important to human health, agriculture, energy, global climate change, ecosystem management, and environmental cleanup and restoration. It is particularly useful for providing direct linkages of microbial genes/populations to ecosystem processes and functions.The ISME Journal (2007) 1, 67-77; doi:10.1038/ismej.2007.2.

GeoChip: a comprehensive microarray for investigating biogeochemical, ecological and environmental processes. Publishing Authors By Initials

Z He,TJ Gentry,CW Schadt,L Wu,J Liebich,SC Chong,Z Huang,W Wu,B Gu,P Jardine,C Criddle,J Zhou,Z He,TJ Gentry,CW Schadt,L Wu,J Liebich,SC Chong,Z Huang,W Wu,B Gu,P Jardine,C Criddle,J Zhou,Z He,TJ Gentry,CW Schadt,L Wu,J Liebich,SC Chong,Z Huang,W Wu,B Gu,P Jardine,C Criddle,J Zhou,

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GeoChip: a comprehensive microarray for investigating biogeochemical, ecological and environmental processes. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: The ISME journal

VOLUME: 1

Page Numbers: 67-77

Journal Abbreviation: ISME J

ISSN: 1751-7362

DAY: 28

MONTH: May

YEAR: 2007

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LANGUAGE: eng

NlmUniqueID: 101301086

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AFFILIATION: [1] 1Department of Botany and Microbiology, Institute for Environmental Genomics, University of Oklahoma, Norman, OK, USA [2] 2Environmental Sciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA.

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