[Genotype combination distribution of 5-site SNPs in 3 subtypes of beta adrenoceptor]

[Genotype combination distribution of 5-site SNPs in 3 subtypes of beta adrenoceptor] Research Abstract Details 

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[Genotype combination distribution of 5-site SNPs in 3 subtypes of beta adrenoceptor] Abstract Text:

Zhengxiang Xie,Yonghong Niu,Houxun Ma,Zhangyong Li,Yuehui Yin,

DNA was extracted from the peripheral venous blood of 338 subjects using BLOOD DNA MINI KIT. The 5 site SNP in 3 subtypes of Beta-AR were genotyped by PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) and allele-specific primer PCR techniques. The genotypes combination distribution of SNP at 5 sites in the 3 subtypes of Beta-AR were determined by clustering analysis technique. The natural combination distribution characteristics for SNP at 5 sites in the 3 subtypes of Beta-AR in 338 subjects were obtained. Sixty-seven combinations types were found. The preceding 5 combinations in the natural combination distribution of the SNP were: (1) The genotype combination of forty subjects was B1-AR S/S49+B1-AR R/R389+B2-AR R/G16+B2-AR Q/E27+B3-AR W/W64, its probability was 11.83%. (2) The genotype combination of thirty-three subjects was B1-AR S/S49+B1-AR R/R389+B2-AR R/G16+B2-AR Q/Q27+B3-AR W/W64, its probability was 9.76%. (3) The genotype combination of nineteen subjects was B1-AR S/S49+B1-AR R/G389+B2-AR R/G16+B2-AR Q/Q27+B3-AR W/W64, its probability was 5.62%. (4) The genotype combination of sixteen subjects was B1-AR S/S49+B1-AR R/G389+B2-AR R/G16+B2-AR Q/E27+B3-AR W/W64, its probability was 4.74%. (5) The genotype combination of thirteen subjects was B1-AR S/G49+B1-AR R/R389+B2-AR R/G16+B2-AR Q/E27+B3-AR W/W64, its probability was 3.85%. The obvious correlations exist among full sample and female or male subgroup, and between female and male subgroups.

[Genotype combination distribution of 5-site SNPs in 3 subtypes of beta adrenoceptor] Publishing Authors By Initials

Z Xie,Y Niu,H Ma,Z Li,Y Yin,

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[Genotype combination distribution of 5-site SNPs in 3 subtypes of beta adrenoceptor] Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Sheng wu yi xue gong cheng xue za zhi = Journal of

VOLUME: 22

Page Numbers: 99-103

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ISSN: 1001-5515

DAY: 14

MONTH: Feb

YEAR: 2005

[Genotype combination distribution of 5-site SNPs in 3 subtypes of beta adrenoceptor] Information

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LANGUAGE: chi

NlmUniqueID: 9426398

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AFFILIATION: Department of Biomedical Engineering in Chongqing University of Medical Sciences, Chongqing 400016, China. bmezxxie@public.cta.cq.cn

Country: China

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MEDLINETA: Sheng Wu Yi Xue Gong Cheng Xue

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