Genomic Changes in Resynthesized Brassica napus and Their Effect on Gene Expression and Phenotype.

Genomic Changes in Resynthesized Brassica napus and Their Effect on Gene Expression and Phenotype. Research Abstract Details 

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Genomic Changes in Resynthesized Brassica napus and Their Effect on Gene Expression and Phenotype. Abstract Text:

Robert T Gaeta,J Chris Pires,Federico Iniguez-Luy,Enrique Leon,Thomas C Osborn,Robert T Gaeta,J Chris Pires,Federico Iniguez-Luy,Enrique Leon,Thomas C Osborn,

Many previous studies have provided evidence for genome changes in polyploids, but there are little data on the overall population dynamics of genome change and whether it causes phenotypic variability. We analyzed genetic, epigenetic, gene expression, and phenotypic changes in approximately 50 resynthesized Brassica napus lines independently derived by hybridizing double haploids of Brassica oleracea and Brassica rapa. A previous analysis of the first generation (S0) found that genetic changes were rare, and cytosine methylation changes were frequent. Our analysis of a later generation found that most S0 methylation changes remained fixed in their S5 progeny, although there were some reversions and new methylation changes. Genetic changes were much more frequent in the S5 generation, occurring in every line with lines normally distributed for number of changes. Genetic changes were detected on 36 of the 38 chromosomes of the S5 allopolyploids and were not random across the genome. DNA fragment losses within lines often occurred at linked marker loci, and most fragment losses co-occurred with intensification of signal from homoeologous markers, indicating that the changes were due to homoeologous nonreciprocal transpositions (HNRTs). HNRTs between chromosomes A1 and C1 initiated in early generations, occurred in successive generations, and segregated, consistent with a recombination mechanism. HNRTs and deletions were correlated with qualitative changes in the expression of specific homoeologous genes and anonymous cDNA amplified fragment length polymorphisms and with phenotypic variation among S5 polyploids. Our data indicate that exchanges among homoeologous chromosomes are a major mechanism creating novel allele combinations and phenotypic variation in newly formed B. napus polyploids.

Genomic Changes in Resynthesized Brassica napus and Their Effect on Gene Expression and Phenotype. Publishing Authors By Initials

RT Gaeta,JC Pires,F Iniguez-Luy,E Leon,TC Osborn,RT Gaeta,JC Pires,F Iniguez-Luy,E Leon,TC Osborn,

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Genomic Changes in Resynthesized Brassica napus and Their Effect on Gene Expression and Phenotype. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: The Plant cell

VOLUME: 19

Page Numbers: 3403-17

Journal Abbreviation: Plant Cell

ISSN: 1040-4651

DAY: 16

MONTH: 11

YEAR: 2007

Genomic Changes in Resynthesized Brassica napus and Their Effect on Gene Expression and Phenotype. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Genomic Changes in Resynthesized Brassica napus and Their Effect on Gene Expression and Phenotype.

AFFILIATION: Department of Agronomy, University of Wisconsin, Madison, Wisconsin.

Country: United States

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MEDLINETA: Plant Cell

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