Genomic and proteomic profiling II: comparative assessment of gene expression profiles in leiomyomas, keloids, and surgically-induced scars.

Genomic and proteomic profiling II: comparative assessment of gene expression profiles in leiomyomas, keloids, and surgically-induced scars. Research Abstract Details 

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Genomic and proteomic profiling II: comparative assessment of gene expression profiles in leiomyomas, keloids, and surgically-induced scars. Abstract Text:

Xiaoping Luo,Qun Pan,Li Liu,Nasser Chegini,

BACKGROUND: Leiomyoma have often been compared to keloids because of their fibrotic characteristic and higher rate of occurrence among African Americans as compared to other ethnic groups. To evaluate such a correlation at molecular level this study comparatively analyzed leiomyomas with keloids, surgical scars and peritoneal adhesions to identify genes that are either commonly and/or individually distinguish these fibrotic disorders despite differences in the nature of their development and growth. METHODS: Microarray gene expression profiling and realtime PCR. RESULTS: The analysis identified 3 to 12% of the genes on the arrays as differentially expressed among these tissues based on P ranking at greater than or equal to 0.005 followed by 2-fold cutoff change selection. Of these genes about 400 genes were identified as differentially expressed in leiomyomas as compared to keloids/incisional scars, and 85 genes as compared to peritoneal adhesions (greater than or equal to 0.01). Functional analysis indicated that the majority of these genes serve as regulators of cell growth (cell cycle/apoptosis), tissue turnover, transcription factors and signal transduction. Of these genes the expression of E2F1, RUNX3, EGR3, TBPIP, ECM-2, ESM1, THBS1, GAS1, ADAM17, CST6, FBLN5, and COL18A was confirmed in these tissues using quantitative realtime PCR based on low-density arrays. CONCLUSION: the results indicated that the molecular feature of leiomyomas is comparable but may be under different tissue-specific regulatory control to those of keloids and differ at the levels rather than tissue-specific expression of selected number of genes functionally regulating cell growth and apoptosis, inflammation, angiogenesis and tissue turnover.

Genomic and proteomic profiling II: comparative assessment of gene expression profiles in leiomyomas, keloids, and surgically-induced scars. Publishing Authors By Initials

X Luo,Q Pan,L Liu,N Chegini,

For similar neoplasms: neoplasms by site: urogenital neoplasms: genital neoplasms, female: uterine neoplasms research abstracts see: neoplasms: neoplasms by site: urogenital neoplasms: genital neoplasms, female: uterine neoplasms research

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Genomic and proteomic profiling II: comparative assessment of gene expression profiles in leiomyomas, keloids, and surgically-induced scars. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Reproductive biology and endocrinology : RB&E

VOLUME: 5

Page Numbers: 35

Journal Abbreviation: Reprod. Biol. Endocrinol.

ISSN: 1477-7827

DAY: 24

MONTH: 08

YEAR: 2007

Genomic and proteomic profiling II: comparative assessment of gene expression profiles in leiomyomas, keloids, and surgically-induced scars. Information

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LANGUAGE: eng

NlmUniqueID: 101153627

Genomic and proteomic profiling II: comparative assessment of gene expression profiles in leiomyomas, keloids, and surgically-induced scars. Keywords Mesh Terms:

KEYWORDS: Uterine Neoplasms

MESH TERMS: metabolism

Chemical & Substance for Abstract: Genomic and proteomic profiling II: comparative assessment of gene expression profiles in leiomyomas, keloids, and surgically-induced scars. Information

Substance Name: RNA, Messenger

Registry Number: 0

Grant and Affiliation Information for Genomic and proteomic profiling II: comparative assessment of gene expression profiles in leiomyomas, keloids, and surgically-induced scars.

AFFILIATION: Department of Obstetrics and Gynecology, University of Florida, College of Medicine, Gainesville, Florida 32610, USA. xiaoping@obgyn.ufl.edu

Country: England

AGENCY: United States NICHD

GRANT: HD37432

ACRONYM: HD

MEDLINETA: Reprod Biol Endocrinol

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