Genomic and metabolic studies of the impact of probiotics on a model gut symbiont and host.

Genomic and metabolic studies of the impact of probiotics on a model gut symbiont and host. Research Abstract Details 

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Genomic and metabolic studies of the impact of probiotics on a model gut symbiont and host. Abstract Text:

Justin L Sonnenburg,Christina T L Chen,Jeffrey I Gordon,

Probiotics are deliberately ingested preparations of live bacterial species that confer health benefits on the host. Many of these species are associated with the fermentation of dairy products. Despite their increasing use, the molecular details of the impact of various probiotic preparations on resident members of the gut microbiota and the host are generally lacking. To address this issue, we colonized germ-free mice with Bacteroides thetaiotaomicron, a prominent component of the adult human gut microbiota, and Bifidobacterium longum, a minor member but a commonly used probiotic. Simultaneous whole genome transcriptional profiling of both bacterial species in their gut habitat and of the intestinal epithelium, combined with mass-spectrometric analysis of habitat-associated carbohydrates, revealed that the presence of B. longum elicits an expansion in the diversity of polysaccharides targeted for degradation by B. thetaiotaomicron (e.g., mannose- and xylose-containing glycans), and induces host genes involved in innate immunity. Although the overall transcriptome expressed by B. thetaiotaomicron when it encounters B. longum in the cecum is dependent upon the genetic background of the mouse (as assessed by a mixed analysis of variance [ANOVA] model of co-colonization experiments performed in NMRI and C57BL/6J animals), B. thetaiotaomicron's expanded capacity to utilize polysaccharides occurs independently of host genotype, and is also observed with a fermented dairy product-associated strain, Lactobacillus casei. This gnotobiotic mouse model provides a controlled case study of how a resident symbiont and a probiotic species adapt their substrate utilization in response to one another, and illustrates both the generality and specificity of the relationship between a host, a component of its microbiota, and intentionally consumed microbial species.

Genomic and metabolic studies of the impact of probiotics on a model gut symbiont and host. Publishing Authors By Initials

JL Sonnenburg,CT Chen,JI Gordon,

For similar carbohydrates: monosaccharides: pentoses: xylose research abstracts see: carbohydrates: monosaccharides: pentoses: xylose research

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MEDLINE DATE:

Genomic and metabolic studies of the impact of probiotics on a model gut symbiont and host. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: PLoS biology

VOLUME: 4

Page Numbers: e413

Journal Abbreviation: PLoS Biol.

ISSN: 1545-7885

DAY: 3

MONTH: Nov

YEAR: 2006

Genomic and metabolic studies of the impact of probiotics on a model gut symbiont and host. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101183755

Genomic and metabolic studies of the impact of probiotics on a model gut symbiont and host. Keywords Mesh Terms:

KEYWORDS: Xylose

MESH TERMS: metabolism

Chemical & Substance for Abstract: Genomic and metabolic studies of the impact of probiotics on a model gut symbiont and host. Information

Substance Name: Mannosidases

Registry Number: EC 3.2.1.-

Grant and Affiliation Information for Genomic and metabolic studies of the impact of probiotics on a model gut symbiont and host.

AFFILIATION: Center for Genome Sciences, Washington University School of Medicine, St. Louis, Missouri, United States of America.

Country: United States

AGENCY: United States NIGMS

GRANT: GM62116

ACRONYM: GM

MEDLINETA: PLoS Biol

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DATABASENAME:

ACCESSION NUMBER: GSE5870

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