Genomewide screens for Escherichia coli genes affecting growth of T7 bacteriophage.

Genomewide screens for Escherichia coli genes affecting growth of T7 bacteriophage. Research Abstract Details 

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Genomewide screens for Escherichia coli genes affecting growth of T7 bacteriophage. Abstract Text:

Udi Qimron,Boriana Marintcheva,Stanley Tabor,Charles C Richardson,

Use of bacteriophages as a therapy for bacterial infection has been attempted over the last century. Such an endeavor requires the elucidation of basic aspects of the host-virus interactions and the resistance mechanisms of the host. Two recently developed bacterial collections now enable a genomewide search of the genetic interactions between Escherichia coli and bacteriophages. We have screened >85% of the E. coli genes for their ability to inhibit growth of T7 phage and >90% of the host genes for their ability to be used by the virus. In addition to identifying all of the known interactions, several other interactions have been identified. E. coli CMP kinase is essential for T7 growth, whereas overexpression of the E. coli uridine/cytidine kinase inhibits T7 growth. Mutations in any one of nine genes that encode enzymes for the synthesis of the E. coli lipopolysaccharide receptor for T7 adsorption leads to T7 resistance. Selection of T7 phage that can recognize these altered receptors has enabled the construction of phage to which the host is 100-fold less resistant.

Genomewide screens for Escherichia coli genes affecting growth of T7 bacteriophage. Publishing Authors By Initials

U Qimron,B Marintcheva,S Tabor,CC Richardson,

For similar biological phenomena, cell phenomena, and immunity: biological phenomena: microbiologic phenomena: virulence research abstracts see: biological phenomena, cell phenomena, and immunity: biological phenomena: microbiologic phenomena: virulence research

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Genomewide screens for Escherichia coli genes affecting growth of T7 bacteriophage. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Proceedings of the National Academy of Sciences of

VOLUME: 103

Page Numbers: 19039-44

Journal Abbreviation: Proc. Natl. Acad. Sci. U.S.A.

ISSN: 0027-8424

DAY: 29

MONTH: 11

YEAR: 2006

Genomewide screens for Escherichia coli genes affecting growth of T7 bacteriophage. Information

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LANGUAGE: eng

NlmUniqueID: 7505876

Genomewide screens for Escherichia coli genes affecting growth of T7 bacteriophage. Keywords Mesh Terms:

KEYWORDS: Virulence

MESH TERMS: genetics

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Grant and Affiliation Information for Genomewide screens for Escherichia coli genes affecting growth of T7 bacteriophage.

AFFILIATION: Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: GM 54397

ACRONYM: GM

MEDLINETA: Proc Natl Acad Sci U S A

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