Genome-wide reprogramming in hybrids of somatic cells and embryonic stem cells.

Genome-wide reprogramming in hybrids of somatic cells and embryonic stem cells. Research Abstract Details 

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Genome-wide reprogramming in hybrids of somatic cells and embryonic stem cells. Abstract Text:

Dominic J Ambrosi,Borko Tanasijevic,Anupinder Kaur,Craig Obergfell,Rachel J O'Neill,Winfried Krueger,Theodore P Rasmussen,

Recent experiments demonstrate that somatic nuclei can be reprogrammed to a pluripotent state when fused to ESCs. The resulting hybrids are pluripotent as judged by developmental assays, but detailed analyses of the underlying molecular-genetic control of reprogrammed transcription in such hybrids are required to better understand fusion-mediated reprogramming. We produced hybrids of mouse ESCs and fibroblasts that, although nearly tetraploid, exhibit characteristics of normal ESCs, including apparent immortality in culture, ESC-like colony morphology, and pluripotency. Comprehensive analysis of the mouse embryonic fibroblast/ESC hybrid transcriptome revealed global patterns of gene expression reminiscent of ESCs. However, combined analysis of variance and hierarchical clustering analyses revealed at least seven distinct classes of differentially regulated genes in comparisons of hybrids, ESCs, and somatic cells. The largest class includes somatic genes that are silenced in hybrids and ESCs, but a smaller class includes genes that are expressed at nearly equivalent levels in hybrids and ESCs that contain many genes implicated in pluripotency and chromatin function. Reprogrammed genes are distributed throughout the genome. Reprogramming events include both transcriptional silencing and activation of genes residing on chromosomes of somatic origin. Somatic/ESC hybrid cell lines resemble their pre-fusion ESC partners in terms of behavior in culture and pluripotency. However, they contain unique expression profiles that are similar but not identical to normal ESCs. ESC fusion-mediated reprogramming provides a tractable system for the investigation of mechanisms of reprogramming. Disclosure of potential conflicts of interest is found at the end of this article.

Genome-wide reprogramming in hybrids of somatic cells and embryonic stem cells. Publishing Authors By Initials

DJ Ambrosi,B Tanasijevic,A Kaur,C Obergfell,RJ O'Neill,W Krueger,TP Rasmussen,

For similar genetic processes: gene expression: transcription, genetic research abstracts see: genetic processes: gene expression: transcription, genetic research

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Genome-wide reprogramming in hybrids of somatic cells and embryonic stem cells. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Stem cells (Dayton, Ohio)

VOLUME: 25

Page Numbers: 1104-13

Journal Abbreviation:

ISSN: 1066-5099

DAY: 1

MONTH: 02

YEAR: 2007

Genome-wide reprogramming in hybrids of somatic cells and embryonic stem cells. Information

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LANGUAGE: eng

NlmUniqueID: 9304532

Genome-wide reprogramming in hybrids of somatic cells and embryonic stem cells. Keywords Mesh Terms:

KEYWORDS: Transcription, Genetic

MESH TERMS: metabolism

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Grant and Affiliation Information for Genome-wide reprogramming in hybrids of somatic cells and embryonic stem cells.

AFFILIATION: Center for Regenerative Biology, 1392 Storrs Road, U-4243 University of Connecticut, Storrs, Connecticut 06269-4243, USA.

Country: United States

AGENCY: United States NIA

GRANT: R01AG23687

ACRONYM: AG

MEDLINETA: Stem Cells

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