Genome-wide mapping of RELA(p65) binding identifies E2F1 as a transcriptional activator recruited by NF-kappaB upon TLR4 activation.

Genome-wide mapping of RELA(p65) binding identifies E2F1 as a transcriptional activator recruited by NF-kappaB upon TLR4 activation. Research Abstract Details 

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Genome-wide mapping of RELA(p65) binding identifies E2F1 as a transcriptional activator recruited by NF-kappaB upon TLR4 activation. Abstract Text:

Ching-Aeng Lim,Fei Yao,Joyce Jing-Yi Wong,Joshy George,Han Xu,Kuo Ping Chiu,Wing-Kin Sung,Leonard Lipovich,Vinsensius B Vega,Joanne Chen,Atif Shahab,Xiao Dong Zhao,Martin Hibberd,Chia-Lin Wei,Bing Lim,Huck-Hui Ng,Yijun Ruan,Keh-Chuang Chin,

NF-kappaB is a key mediator of inflammation. Here, we mapped the genome-wide loci bound by the RELA subunit of NF-kappaB in lipopolysaccharide (LPS)-stimulated human monocytic cells, and together with global gene expression profiling, found an overrepresentation of the E2F1-binding motif among RELA-bound loci associated with NF-kappaB target genes. Knockdown of endogenous E2F1 impaired the LPS inducibility of the proinflammatory cytokines CCL3(MIP-1alpha), IL23A(p19), TNF-alpha, and IL1-beta. Upon LPS stimulation, E2F1 is rapidly recruited to the promoters of these genes along with p50/RELA heterodimer via a mechanism that is dependent on NF-kappaB activation. Together with the observation that E2F1 physically interacts with p50/RELA in LPS-stimulated cells, our findings suggest that NF-kappaB recruits E2F1 to fully activate the transcription of NF-kappaB target genes. Global gene expression profiling subsequently revealed a spectrum of NF-kappaB target genes that are positively regulated by E2F1, further demonstrating the critical role of E2F1 in the Toll-like receptor 4 pathway.

Genome-wide mapping of RELA(p65) binding identifies E2F1 as a transcriptional activator recruited by NF-kappaB upon TLR4 activation. Publishing Authors By Initials

CA Lim,F Yao,JJ Wong,J George,H Xu,KP Chiu,WK Sung,L Lipovich,VB Vega,J Chen,A Shahab,XD Zhao,M Hibberd,CL Wei,B Lim,HH Ng,Y Ruan,KC Chin,

For similar proteins: dna-binding proteins: nf-kappa b: transcription factor rela research abstracts see: proteins: dna-binding proteins: nf-kappa b: transcription factor rela research

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Genome-wide mapping of RELA(p65) binding identifies E2F1 as a transcriptional activator recruited by NF-kappaB upon TLR4 activation. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Molecular cell

VOLUME: 27

Page Numbers: 622-35

Journal Abbreviation: Mol. Cell

ISSN: 1097-2765

DAY: 17

MONTH: Aug

YEAR: 2007

Genome-wide mapping of RELA(p65) binding identifies E2F1 as a transcriptional activator recruited by NF-kappaB upon TLR4 activation. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9802571

Genome-wide mapping of RELA(p65) binding identifies E2F1 as a transcriptional activator recruited by NF-kappaB upon TLR4 activation. Keywords Mesh Terms:

KEYWORDS: Transcription Factor RelA

MESH TERMS: metabolism

Chemical & Substance for Abstract: Genome-wide mapping of RELA(p65) binding identifies E2F1 as a transcriptional activator recruited by NF-kappaB upon TLR4 activation. Information

Substance Name: Transcription Factor RelA

Registry Number: 0

Grant and Affiliation Information for Genome-wide mapping of RELA(p65) binding identifies E2F1 as a transcriptional activator recruited by NF-kappaB upon TLR4 activation.

AFFILIATION: Laboratory of Immunology and Virology, Genome Institute of Singapore, 138672 Singapore.

Country: United States

AGENCY: United States NHGRI

GRANT: R01HG003521-01

ACRONYM: HG

MEDLINETA: Mol Cell

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