Genome of Methylobacillus flagellatus, molecular basis for obligate methylotrophy, and polyphyletic origin of methylotrophy.

Genome of Methylobacillus flagellatus, molecular basis for obligate methylotrophy, and polyphyletic origin of methylotrophy. Research Abstract Details 

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Genome of Methylobacillus flagellatus, molecular basis for obligate methylotrophy, and polyphyletic origin of methylotrophy. Abstract Text:

Ludmila Chistoserdova,Alla Lapidus,Cliff Han,Lynne Goodwin,Liz Saunders,Tom Brettin,Roxanne Tapia,Paul Gilna,Susan Lucas,Paul M Richardson,Mary E Lidstrom,

Along with methane, methanol and methylated amines represent important biogenic atmospheric constituents; thus, not only methanotrophs but also nonmethanotrophic methylotrophs play a significant role in global carbon cycling. The complete genome of a model obligate methanol and methylamine utilizer, Methylobacillus flagellatus (strain KT) was sequenced. The genome is represented by a single circular chromosome of approximately 3 Mbp, potentially encoding a total of 2,766 proteins. Based on genome analysis as well as the results from previous genetic and mutational analyses, methylotrophy is enabled by methanol and methylamine dehydrogenases and their specific electron transport chain components, the tetrahydromethanopterin-linked formaldehyde oxidation pathway and the assimilatory and dissimilatory ribulose monophosphate cycles, and by a formate dehydrogenase. Some of the methylotrophy genes are present in more than one (identical or nonidentical) copy. The obligate dependence on single-carbon compounds appears to be due to the incomplete tricarboxylic acid cycle, as no genes potentially encoding alpha-ketoglutarate, malate, or succinate dehydrogenases are identifiable. The genome of M. flagellatus was compared in terms of methylotrophy functions to the previously sequenced genomes of three methylotrophs, Methylobacterium extorquens (an alphaproteobacterium, 7 Mbp), Methylibium petroleiphilum (a betaproteobacterium, 4 Mbp), and Methylococcus capsulatus (a gammaproteobacterium, 3.3 Mbp). Strikingly, metabolically and/or phylogenetically, the methylotrophy functions in M. flagellatus were more similar to those in M. capsulatus and M. extorquens than to the ones in the more closely related M. petroleiphilum species, providing the first genomic evidence for the polyphyletic origin of methylotrophy in Betaproteobacteria.

Genome of Methylobacillus flagellatus, molecular basis for obligate methylotrophy, and polyphyletic origin of methylotrophy. Publishing Authors By Initials

L Chistoserdova,A Lapidus,C Han,L Goodwin,L Saunders,T Brettin,R Tapia,P Gilna,S Lucas,PM Richardson,ME Lidstrom,

For similar investigative techniques: genetic techniques: sequence analysis: sequence analysis, dna research abstracts see: investigative techniques: genetic techniques: sequence analysis: sequence analysis, dna research

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Genome of Methylobacillus flagellatus, molecular basis for obligate methylotrophy, and polyphyletic origin of methylotrophy. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Journal of bacteriology

VOLUME: 189

Page Numbers: 4020-7

Journal Abbreviation: J. Bacteriol.

ISSN: 0021-9193

DAY: 6

MONTH: 04

YEAR: 2007

Genome of Methylobacillus flagellatus, molecular basis for obligate methylotrophy, and polyphyletic origin of methylotrophy. Information

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LANGUAGE: eng

NlmUniqueID: 2985120

Genome of Methylobacillus flagellatus, molecular basis for obligate methylotrophy, and polyphyletic origin of methylotrophy. Keywords Mesh Terms:

KEYWORDS: Sequence Analysis, DNA

MESH TERMS: metabolism

Chemical & Substance for Abstract: Genome of Methylobacillus flagellatus, molecular basis for obligate methylotrophy, and polyphyletic origin of methylotrophy. Information

Substance Name: methylamine

Registry Number: 74-89-5

Grant and Affiliation Information for Genome of Methylobacillus flagellatus, molecular basis for obligate methylotrophy, and polyphyletic origin of methylotrophy.

AFFILIATION: University of Washington, Seattle, USA. milachis@u.washington.edu

Country: United States

AGENCY: United States NIGMS

GRANT: 5R01 GM 58933

ACRONYM: GM

MEDLINETA: J Bacteriol

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