Genetic mapping of the pear scab resistance gene Vnk of Japanese pear cultivar Kinchaku.

Genetic mapping of the pear scab resistance gene Vnk of Japanese pear cultivar Kinchaku. Research Abstract Details 

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Genetic mapping of the pear scab resistance gene Vnk of Japanese pear cultivar Kinchaku. Abstract Text:

S Terakami,M Shoda,Y Adachi,T Gonai,M Kasumi,Y Sawamura,H Iketani,K Kotobuki,A Patocchi,C Gessler,T Hayashi,T Yamamoto,

Pear scab (caused by Venturia nashicola) is one of the most harmful diseases of pears, especially Japanese and Chinese pear species. The molecular identification and early selection of resistant plants could greatly improve pear breeding. We have identified the position of the scab resistance gene, designated Vnk in an indigenous Japanese pear cultivar Kinchaku, within the pear genome by using simple sequence repeat (SSR) markers derived from pear and apple. The position of Vnk was identified in the central region of linkage group 1 of Kinchaku. Several amplified fragment length polymorphism (AFLP) markers linked to Vnk were obtained by bulked segregant analysis. Among them, the AFLP marker closest to Vnk was converted into a sequence tagged site (STS) marker. Four random amplified polymorphic DNA (RAPD) markers previously found to be loosely associated with Vnk (Iketani et al. 2001) were successfully converted into STS markers. Six markers (one SSR Hi02c07 and five STSs converted from AFLP and RAPD) showed tight linkages to Vnk, being mapped with distances ranging from 2.4 to 12.4 cM. The SSR CH-Vf2, which was isolated from a BAC clone of the contig containing the apple scab gene Vf, was mapped at the bottom of linkage group 1 in Kinchaku, suggesting that the Vnk and Vf loci are located in different genomic regions of the same homologous linkage group.

Genetic mapping of the pear scab resistance gene Vnk of Japanese pear cultivar Kinchaku. Publishing Authors By Initials

S Terakami,M Shoda,Y Adachi,T Gonai,M Kasumi,Y Sawamura,H Iketani,K Kotobuki,A Patocchi,C Gessler,T Hayashi,T Yamamoto,

For similar plants: plant families and groups: angiosperms: rosales: rosaceae: pyrus research abstracts see: plants: plant families and groups: angiosperms: rosales: rosaceae: pyrus research

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Genetic mapping of the pear scab resistance gene Vnk of Japanese pear cultivar Kinchaku. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: TAG. Theoretical and applied genetics. Theoretisch

VOLUME: 113

Page Numbers: 743-52

Journal Abbreviation: Theor. Appl. Genet.

ISSN: 0040-5752

DAY: 13

MONTH: 07

YEAR: 2006

Genetic mapping of the pear scab resistance gene Vnk of Japanese pear cultivar Kinchaku. Information

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LANGUAGE: eng

NlmUniqueID: 145600

Genetic mapping of the pear scab resistance gene Vnk of Japanese pear cultivar Kinchaku. Keywords Mesh Terms:

KEYWORDS: Pyrus

MESH TERMS: microbiology

Chemical & Substance for Abstract: Genetic mapping of the pear scab resistance gene Vnk of Japanese pear cultivar Kinchaku. Information

Substance Name: Genetic Markers

Registry Number: 0

Grant and Affiliation Information for Genetic mapping of the pear scab resistance gene Vnk of Japanese pear cultivar Kinchaku.

AFFILIATION: Advanced Agricultural Technology and Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-8605, Japan.

Country: Germany

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MEDLINETA: Theor Appl Genet

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