Genetic Interactions between an Essential 3' cis-Acting RNA Pseudoknot, Replicase Gene Products, and the Extreme 3' End of the Mouse Coronavirus Genome.

Genetic Interactions between an Essential 3' cis-Acting RNA Pseudoknot, Replicase Gene Products, and the Extreme 3' End of the Mouse Coronavirus Genome. Research Abstract Details 

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Genetic Interactions between an Essential 3' cis-Acting RNA Pseudoknot, Replicase Gene Products, and the Extreme 3' End of the Mouse Coronavirus Genome. Abstract Text:

Roland ,Timothy B Miller,Scott J Goebel,Volker Thiel,Paul S Masters,Roland ,Timothy B Miller,Scott J Goebel,Volker Thiel,Paul S Masters,Roland Züst,Timothy B Miller,Scott J Goebel,Volker Thiel,Paul S Masters,

The upstream end of the 3' untranslated region (UTR) of the mouse hepatitis virus genome contains two essential and overlapping RNA secondary structures, a bulged stem-loop and a pseudoknot, which have been proposed to be elements of a molecular switch that is critical for viral RNA synthesis. It has previously been shown that a particular six-base insertion in loop 1 of the pseudoknot is extremely deleterious to the virus. We have now isolated multiple independent second-site revertants of the loop 1 insertion mutant, and we used reverse-genetics methods to confirm the identities of suppressor mutations that could compensate for the original insertion. The suppressors were localized to two separate regions of the genome. Members of one class of suppressor were mapped to the portions of gene 1 that encode nsp8 and nsp9, thereby providing the first evidence for specific interactions between coronavirus replicase gene products and a cis-acting genomic RNA element. The second class of suppressor was mapped to the extreme 3' end of the genome, a result which pointed to the existence of a direct base-pairing interaction between loop 1 of the pseudoknot and the genomic terminus. The latter finding was strongly supported by phylogenetic evidence and by the construction of a deletion mutant that reduced the 3' UTR to its minimal essential elements. Taken together, the interactions revealed by the two classes of suppressors suggest a model for the initiation of coronavirus negative-strand RNA synthesis.

Genetic Interactions between an Essential 3' cis-Acting RNA Pseudoknot, Replicase Gene Products, and the Extreme 3' End of the Mouse Coronavirus Genome. Publishing Authors By Initials

R ,TB Miller,SJ Goebel,V Thiel,PS Masters,R ,TB Miller,SJ Goebel,V Thiel,PS Masters,R Züst,TB Miller,SJ Goebel,V Thiel,PS Masters,

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Genetic Interactions between an Essential 3' cis-Acting RNA Pseudoknot, Replicase Gene Products, and the Extreme 3' End of the Mouse Coronavirus Genome. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of virology

VOLUME: 82

Page Numbers: 1214-28

Journal Abbreviation: J. Virol.

ISSN: 1098-5514

DAY: 21

MONTH: 11

YEAR: 2007

Genetic Interactions between an Essential 3' cis-Acting RNA Pseudoknot, Replicase Gene Products, and the Extreme 3' End of the Mouse Coronavirus Genome. Information

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LANGUAGE: eng

NlmUniqueID: 113724

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Grant and Affiliation Information for Genetic Interactions between an Essential 3' cis-Acting RNA Pseudoknot, Replicase Gene Products, and the Extreme 3' End of the Mouse Coronavirus Genome.

AFFILIATION: David Axelrod Institute, Wadsworth Center, NYSDOH, New Scotland Avenue, P.O. Box 22002, Albany, New York 12201-2002. masters@wadsworth.org.

Country: United States

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MEDLINETA: J Virol

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