Genetic exchange between homeologous sequences in mammalian chromosomes is averted by local homology requirements for initiation and resolution of recombination.

Genetic exchange between homeologous sequences in mammalian chromosomes is averted by local homology requirements for initiation and resolution of recombination. Research Abstract Details 

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Genetic exchange between homeologous sequences in mammalian chromosomes is averted by local homology requirements for initiation and resolution of recombination. Abstract Text:

Derek Yang,Edie B Goldsmith,Yunfu Lin,Barbara Criscuolo Waldman,Vimala Kaza,Alan S Waldman,

We examined the mechanism by which recombination between imperfectly matched sequences (homeologous recombination) is suppressed in mammalian chromosomes. DNA substrates were constructed, each containing a thymidine kinase (tk) gene disrupted by insertion of an XhoI linker and referred to as a "recipient" gene. Each substrate also contained one of several "donor" tk sequences that could potentially correct the recipient gene via recombination. Each donor sequence either was perfectly homologous to the recipient gene or contained homeologous sequence sharing only 80% identity with the recipient gene. Mouse Ltk(-) fibroblasts were stably transfected with the various substrates and tk(+) segregants produced via intrachromosomal recombination were recovered. We observed exclusion of homeologous sequence from gene conversion tracts when homeologous sequence was positioned adjacent to homologous sequence in the donor but not when homeologous sequence was surrounded by homology in the donor. Our results support a model in which homeologous recombination in mammalian chromosomes is suppressed by a nondestructive dismantling of mismatched heteroduplex DNA (hDNA) intermediates. We suggest that mammalian cells do not dismantle mismatched hDNA by responding to mismatches in hDNA per se but rather rejection of mismatched hDNA appears to be driven by a requirement for localized homology for resolution of recombination.

Genetic exchange between homeologous sequences in mammalian chromosomes is averted by local homology requirements for initiation and resolution of recombination. Publishing Authors By Initials

D Yang,EB Goldsmith,Y Lin,BC Waldman,V Kaza,AS Waldman,

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Genetic exchange between homeologous sequences in mammalian chromosomes is averted by local homology requirements for initiation and resolution of recombination. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Genetics

VOLUME: 174

Page Numbers: 135-44

Journal Abbreviation:

ISSN: 0016-6731

DAY: 2

MONTH: 07

YEAR: 2006

Genetic exchange between homeologous sequences in mammalian chromosomes is averted by local homology requirements for initiation and resolution of recombination. Information

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LANGUAGE: eng

NlmUniqueID: 374636

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Grant and Affiliation Information for Genetic exchange between homeologous sequences in mammalian chromosomes is averted by local homology requirements for initiation and resolution of recombination.

AFFILIATION: Department of Biological Sciences, University of South Carolina, Columbia, South Carolina 29208, USA.

Country: United States

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MEDLINETA: Genetics

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