Genetic evidence for parallel pathways of chaperone activity in the periplasm of Escherichia coli.

Genetic evidence for parallel pathways of chaperone activity in the periplasm of Escherichia coli. Research Abstract Details 

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Genetic evidence for parallel pathways of chaperone activity in the periplasm of Escherichia coli. Abstract Text:

A E Rizzitello,J R Harper,T J Silhavy,

The periplasm of Escherichia coli contains many proteins proposed to have redundant functions in protein folding. Using depletion analysis, we directly demonstrated that null mutations in skp and surA, as well as in degP and surA, result in synthetic phenotypes, suggesting that Skp, SurA, and DegP are functionally redundant. The Deltaskp surA::kan combination has a bacteriostatic effect and leads to filamentation, while the degP::Tn10 surA::kan combination is bactericidal. The steady-state levels of several envelope proteins are greatly reduced upon depletion of a wild-type copy of surA in both instances. We suggest that the functional redundancy of Skp, SurA, and DegP lies in the periplasmic chaperone activity. Taken together, our data support a model in which the periplasm of E. coli contains parallel pathways for chaperone activity. In particular, we propose that Skp and DegP are components of the same pathway and that SurA is a component of a separate pathway. The loss of either pathway has minimal effects on the cell, while the loss of both pathways results in the synthetic phenotypes observed.

Genetic evidence for parallel pathways of chaperone activity in the periplasm of Escherichia coli. Publishing Authors By Initials

AE Rizzitello,JR Harper,TJ Silhavy,

For similar proteins: membrane proteins: receptors, cell surface: receptors, virus research abstracts see: proteins: membrane proteins: receptors, cell surface: receptors, virus research

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Genetic evidence for parallel pathways of chaperone activity in the periplasm of Escherichia coli. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Journal of bacteriology

VOLUME: 183

Page Numbers: 6794-800

Journal Abbreviation: J. Bacteriol.

ISSN: 0021-9193

DAY: 27

MONTH: Dec

YEAR: 2001

Genetic evidence for parallel pathways of chaperone activity in the periplasm of Escherichia coli. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985120

Genetic evidence for parallel pathways of chaperone activity in the periplasm of Escherichia coli. Keywords Mesh Terms:

KEYWORDS: Receptors, Virus

MESH TERMS: analysis

Chemical & Substance for Abstract: Genetic evidence for parallel pathways of chaperone activity in the periplasm of Escherichia coli. Information

Substance Name: Peptidylprolyl Isomerase

Registry Number: EC 5.2.1.8

Grant and Affiliation Information for Genetic evidence for parallel pathways of chaperone activity in the periplasm of Escherichia coli.

AFFILIATION: Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: GM 34821

ACRONYM: GM

MEDLINETA: J Bacteriol

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