Genetic and molecular properties of human and rat renin-binding proteins with reference to the function of the leucine zipper motif.

Genetic and molecular properties of human and rat renin-binding proteins with reference to the function of the leucine zipper motif. Research Abstract Details 

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Genetic and molecular properties of human and rat renin-binding proteins with reference to the function of the leucine zipper motif. Abstract Text:

H Inoue,S Takahashi,K Fukui,Y Miyake,

The presence of a leucine zipper motif was recognized in the deduced amino acid sequences of human and rat renin-binding proteins (RnBPs) on cloning and sequence analysis of the RnBP cDNAs. The in vitro synthesized RnBPs, with the respective cDNAs, formed heterodimers with porcine renin and homodimers. On comparison of these properties with those of porcine RnBP, the leucine zipper motif was suggested to be a functional domain common to animal RnBPs. In addition to the motif, a hydrophobic domain adjacent to the motif and 10 cysteine residues were also well conserved in the three RnBPs. Moreover, about 85% of their amino acid sequences were identical. The RnBP mRNAs were expressed in the kidneys as the same size of 1.5-kb and the genes are suggested to exist as single copies in the genomes. Despite the high similarities in genetic and molecular properties, the molecular weights of human and rat RnBPs were 43,000, which is 1,000 larger than that of porcine RnBP. The immunoreactivities of human and rat RnBPs toward anti-porcine RnBP antiserum were 88 and 8% that of porcine RnBP, respectively, and the affinities of the two RnBPs for porcine renin were remarkably less than that of porcine RnBP. Moreover, the human and rat RnBP homodimers were partly dissociated under the conditions under which porcine RnBP existed as a dimer. These results indicate distinct differences in the molecular properties among the three RnBPs, in spite of their being highly similar structurally and functionally.

Genetic and molecular properties of human and rat renin-binding proteins with reference to the function of the leucine zipper motif. Publishing Authors By Initials

H Inoue,S Takahashi,K Fukui,Y Miyake,

For similar animals: chordata: vertebrates: mammals: artiodactyla: swine research abstracts see: animals: chordata: vertebrates: mammals: artiodactyla: swine research

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Genetic and molecular properties of human and rat renin-binding proteins with reference to the function of the leucine zipper motif. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 110

Page Numbers: 493-500

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Oct

YEAR: 1991

Genetic and molecular properties of human and rat renin-binding proteins with reference to the function of the leucine zipper motif. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376600

Genetic and molecular properties of human and rat renin-binding proteins with reference to the function of the leucine zipper motif. Keywords Mesh Terms:

KEYWORDS: Swine

MESH TERMS: metabolism

Chemical & Substance for Abstract: Genetic and molecular properties of human and rat renin-binding proteins with reference to the function of the leucine zipper motif. Information

Substance Name: Renbp protein, rat

Registry Number: EC 5.1.3.8

Grant and Affiliation Information for Genetic and molecular properties of human and rat renin-binding proteins with reference to the function of the leucine zipper motif.

AFFILIATION: Department of Biochemistry, National Cardiovascular Center Research Institute, Osaka.

Country: JAPAN

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MEDLINETA: J Biochem

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ACCESSION NUMBER: S78724

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