Genetic, age and light mediated effects on crystallin protein expression in the retina.

Genetic, age and light mediated effects on crystallin protein expression in the retina. Research Abstract Details 

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Genetic, age and light mediated effects on crystallin protein expression in the retina. Abstract Text:

Daniel Organisciak,Ruth Darrow,Xiaorong Gu,Linda Barsalou,John W Crabb,

To probe for possible relationships between retinal crystallins and retinal degenerations, protein expression was compared in normal Sprague-Dawley rats, treated or not with intense light, Royal College of Surgeons (RCS) rats and transgenic rats expressing rhodopsin mutations. Rats were reared in dim cyclic light for 21-75 days. Photoreceptor cell DNA levels were determined at various ages to assess the rates of visual cell loss. 1D- and 2D-gel electrophoresis was used to profile retinal protein expression. Crystallins were identified by western analysis and by tandem mass spectrometry. In normal rat retinas, alpha, beta and gamma crystallins were present, although alphaA- and gamma-crystallins exhibited some increase with age. As measured by DNA levels, the rate of genetically induced photoreceptor cell loss was greater in rats with faster degenerating retinas (RCS, S334-ter Line 4, P23H Line 3) than in rats with slower degenerating retinas (S334-ter Line 9, P23H Line 2). In genetic models of retinal degeneration increased levels of immunoreactivity for all crystallins, especially alphaA-insert, correlated with the different rates of photoreceptor loss. In the light induced degeneration model alphaA-insert was unchanged, truncated alphaB-crystallin levels were increased and gamma-crystallins were greatly reduced. In the RCS rat retina 16 different crystallins were identified. Our data suggests that an increase in crystallin expression occurs during various retinal degenerations and that the increases may be related to the severity, type and onset of retinal degeneration.

Genetic, age and light mediated effects on crystallin protein expression in the retina. Publishing Authors By Initials

D Organisciak,R Darrow,X Gu,L Barsalou,JW Crabb,

For similar proteins: eye proteins: opsin: rhodopsin research abstracts see: proteins: eye proteins: opsin: rhodopsin research

PUBMED ID PMID:

MEDLINE DATE: 2006 Jul-Aug

Genetic, age and light mediated effects on crystallin protein expression in the retina. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Photochemistry and photobiology

VOLUME: 82

Page Numbers: 1088-96

Journal Abbreviation: Photochem. Photobiol.

ISSN: 0031-8655

DAY: 3

MONTH: 12

YEAR: 2007

Genetic, age and light mediated effects on crystallin protein expression in the retina. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376425

Genetic, age and light mediated effects on crystallin protein expression in the retina. Keywords Mesh Terms:

KEYWORDS: Rhodopsin

MESH TERMS: genetics

Chemical & Substance for Abstract: Genetic, age and light mediated effects on crystallin protein expression in the retina. Information

Substance Name: Rhodopsin

Registry Number: 9009-81-8

Grant and Affiliation Information for Genetic, age and light mediated effects on crystallin protein expression in the retina.

AFFILIATION: Petticrew Research Laboratory, School of Medicine, Wright State University, Dayton, OH, USA. dto@wright.edu

Country: United States

AGENCY: United States NEI

GRANT: EY15638

ACRONYM: EY

MEDLINETA: Photochem Photobiol

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