Generation of temperature-sensitive human metapneumovirus strains that provide protective immunity in hamsters.

Generation of temperature-sensitive human metapneumovirus strains that provide protective immunity in hamsters. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Generation of temperature-sensitive human metapneumovirus strains that provide protective immunity in hamsters. Abstract Text:

Human metapneumovirus (HMPV) causes acute respiratory tract illness primarily in young children, immunocompromised individuals and the elderly. Vaccines would be desirable to prevent severe illnesses in these risk groups. Here, we describe the generation and evaluation of cold-passage (cp) temperature-sensitive (ts) HMPV strains as vaccine candidates. Repeated passage of HMPV at low temperatures in Vero cells resulted in the accumulation of mutations in the viral genome. Introduction of these mutations in a recombinant HMPV by reverse genetics resulted in a ts-phenotype, judged on the decreased shut-off temperature for virus replication in vitro. As an alternative approach, three previously described cp-respiratory syncytial virus (cp-HRSV) mutations were introduced in a recombinant HMPV, which also resulted in a low shut-off temperature in vitro. Replication of these ts-viruses containing either the cp-HMPV or cp-HRSV mutations was reduced in the upper respiratory tract (URT) and undetectable in the lower respiratory tract (LRT) of hamsters. Nevertheless, high titres of HMPV-specific antibodies were induced by both ts-viruses. Upon immunization with the ts-viruses, the LRT of hamsters were completely protected against challenge infection with a heterologous HMPV strain, and URT viral titres were reduced by 10 000-fold. In conclusion, we provide proof-of-principle for two candidate live-attenuated HMPV vaccines that induce cross-protective immunity to prevent infection of the LRT in Syrian golden hamsters. Further mapping of the molecular determinants of attenuation of HMPV should be the subject of future studies.

Generation of temperature-sensitive human metapneumovirus strains that provide protective immunity in hamsters. Publishing Authors By Initials

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Generation of temperature-sensitive human metapneumovirus strains that provide protective immunity in hamsters. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: The Journal of general virology

VOLUME: 89

Page Numbers: 1553-62

Journal Abbreviation: J. Gen. Virol.

ISSN: 0022-1317

DAY: 18

MONTH: Jul

YEAR: 2008

Generation of temperature-sensitive human metapneumovirus strains that provide protective immunity in hamsters. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 77340

Generation of temperature-sensitive human metapneumovirus strains that provide protective immunity in hamsters. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Generation of temperature-sensitive human metapneumovirus strains that provide protective immunity in hamsters. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Generation of temperature-sensitive human metapneumovirus strains that provide protective immunity in hamsters.

AFFILIATION: Department of Virology, Erasmus Medical Center, Rotterdam, The Netherlands.

Country: England

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Gen Virol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Generation of temperature-sensitive human metapneumovirus strains that provide protective immunity in hamsters Related Publications

• Antigenic and genetic evolution of swine influenza A (H3N2) viruses in Europe.
• Emerging influenza.
• Generation of temperature-sensitive human metapneumovirus strains that provide protective immunity in hamsters.
• Immunogenicity and efficacy of two candidate human metapneumovirus vaccines in cynomolgus macaques.
• Specificity and functional interaction of the polymerase complex proteins of human and avian metapneumoviruses.
• Vaccination approaches to combat human metapneumovirus lower respiratory tract infections.