Generation of rat mutants using a coat color-tagged Sleeping Beauty transposon system.

Generation of rat mutants using a coat color-tagged Sleeping Beauty transposon system. Research Abstract Details 

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Generation of rat mutants using a coat color-tagged Sleeping Beauty transposon system. Abstract Text:

Baisong Lu,Aron M Geurts,Christophe Poirier,Deborah C Petit,Wilbur Harrison,Paul A Overbeek,Colin E Bishop,

A significant barrier to exploiting the full potential of the rat as a biomedical model is the lack of tools to easily modify its germline. Here we show that a tyrosinase-tagged Sleeping Beauty transposon can be used as a simple, efficient method to generate rat mutants in vivo. By making two lines of transgenic rats, one carrying the transposon and another expressing the transposase in germ cells, we are able to obtain bigenic males in which transposition occurs in the germ cells. We show that transposition leads to the appearance of new coat colors in the offspring. Using such bigenic males, we obtained an average of 1.2 transpositions per gamete and identified 19 intragenic integration events among 96 transposition sites that were sequenced. In addition, gene trapping was confirmed and rats with evidence for transposon-induced dominant ocular anomalies were identified. These data suggest that the modified Sleeping Beauty transposon represents a powerful new tool for producing molecularly defined mutagenesis in the rat.

Generation of rat mutants using a coat color-tagged Sleeping Beauty transposon system. Publishing Authors By Initials

B Lu,AM Geurts,C Poirier,DC Petit,W Harrison,PA Overbeek,CE Bishop,

For similar animals: chordata: vertebrates: mammals: rodentia: muridae: murinae: rats research abstracts see: animals: chordata: vertebrates: mammals: rodentia: muridae: murinae: rats research

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Generation of rat mutants using a coat color-tagged Sleeping Beauty transposon system. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Mammalian genome : official journal of the Interna

VOLUME: 18

Page Numbers: 338-46

Journal Abbreviation: Mamm. Genome

ISSN: 0938-8990

DAY: 8

MONTH: 06

YEAR: 2007

Generation of rat mutants using a coat color-tagged Sleeping Beauty transposon system. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9100916

Generation of rat mutants using a coat color-tagged Sleeping Beauty transposon system. Keywords Mesh Terms:

KEYWORDS: Rats

MESH TERMS: genetics

Chemical & Substance for Abstract: Generation of rat mutants using a coat color-tagged Sleeping Beauty transposon system. Information

Substance Name: Monophenol Monooxygenase

Registry Number: EC 1.14.18.1

Grant and Affiliation Information for Generation of rat mutants using a coat color-tagged Sleeping Beauty transposon system.

AFFILIATION: Department of Obstetrics and Gynecology, Baylor College of Medicine, Houston, Texas 77030, USA.

Country: United States

AGENCY: United States NCRR

GRANT: R21RR021947

ACRONYM: RR

MEDLINETA: Mamm Genome

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