Generation of a transgenic rice seed-based edible vaccine against house dust mite allergy.

Generation of a transgenic rice seed-based edible vaccine against house dust mite allergy. Research Abstract Details 

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Generation of a transgenic rice seed-based edible vaccine against house dust mite allergy. Abstract Text:

Lijun Yang,Hiroyuki Kajiura,Kazuya Suzuki,Sakiko Hirose,Kazuhito Fujiyama,Fumio Takaiwa,Lijun Yang,Hiroyuki Kajiura,Kazuya Suzuki,Sakiko Hirose,Kazuhito Fujiyama,Fumio Takaiwa,

As an alternative approach to conventional allergen-specific immunotherapy, transgenic rice seed expressing a major house dust mite (HDM) allergen, Der p 1, was developed as an edible vaccine. The C-terminal KDEL-tagged Der p 1 allergen specifically accumulated in seed endosperm tissue under the control of the endosperm-specific GluB1 promoter. Der p 1 reached a maximum concentration of 58 microg/grain and was deposited in the endoplasmic reticulum (ER)-derived protein body I (PB-I). Plant-derived Der p 1 was posttranslationally modified with high-mannose-type glycan structures. Glycosylated Der p 1 displayed reduced IgE binding capacity in comparison with its unglycosylated counterpart in vitro. Our results indicate that transgenic Der p 1 rice seeds are a safe, potential oral delivery vaccine for the treatment of HDM allergy.

Generation of a transgenic rice seed-based edible vaccine against house dust mite allergy. Publishing Authors By Initials

L Yang,H Kajiura,K Suzuki,S Hirose,K Fujiyama,F Takaiwa,L Yang,H Kajiura,K Suzuki,S Hirose,K Fujiyama,F Takaiwa,

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Generation of a transgenic rice seed-based edible vaccine against house dust mite allergy. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Biochemical and biophysical research communication

VOLUME: 365

Page Numbers: 334-9

Journal Abbreviation: Biochem. Biophys. Res. Commun.

ISSN: 1090-2104

DAY: 5

MONTH: 11

YEAR: 2007

Generation of a transgenic rice seed-based edible vaccine against house dust mite allergy. Information

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LANGUAGE: eng

NlmUniqueID: 372516

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AFFILIATION: Transgenic Crop Research and Development Center, National Institute of Agrobiological Sciences, Kannondai 2-1-2, Tsukuba, Ibaraki 305-8602, Japan.

Country: United States

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MEDLINETA: Biochem Biophys Res Commun

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