Generation, characterization and biological activity of CCL2 (MCP-1/JE) and CCL12 (MCP-5) specific antibodies.

Generation, characterization and biological activity of CCL2 (MCP-1/JE) and CCL12 (MCP-5) specific antibodies. Research Abstract Details 

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Generation, characterization and biological activity of CCL2 (MCP-1/JE) and CCL12 (MCP-5) specific antibodies. Abstract Text:

Ping Tsui,Anuk Das,Brian Whitaker,Mark Tornetta,Nicole Stowell,Prabakaran Kesavan,Elisabeth Kaiser,Eilyn R Lacy,Li Yan,Linda A Snyder,Raymond Sweet,

The human CCL2 chemokine is implicated in many chronic inflammatory conditions. In the mouse, there are two CCL2 homologues, CCL2 (MCP-1/JE) and CCL12 (MCP-5). Both are potent monocyte chemoattractants and bind to and activate the same receptor, CCR2. The overlapping activities of these chemokines complicate the design of mouse model studies that are intended to mimic human disease. To study the roles of CCL2 and CCL12, we generated neutralizing antibodies specific to each chemokine. Consistent with binding and affinity analyses, the antibodies specifically inhibited CCL2- or CCL12- mediated Ca;{2+} mobilization in THP-1 cells. When tested in nude mice bearing human PANC-1 pancreatic tumor cells in Matrigeltrade mark plugs, CCL2 and CCL12 antibodies potently inhibited tumor angiogenesis, indicating that both CCL2 and CCL12 may contribute to tumor angiogenesis.

Generation, characterization and biological activity of CCL2 (MCP-1/JE) and CCL12 (MCP-5) specific antibodies. Publishing Authors By Initials

P Tsui,A Das,B Whitaker,M Tornetta,N Stowell,P Kesavan,E Kaiser,ER Lacy,L Yan,LA Snyder,R Sweet,

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Generation, characterization and biological activity of CCL2 (MCP-1/JE) and CCL12 (MCP-5) specific antibodies. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Human antibodies

VOLUME: 16

Page Numbers: 117-25

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ISSN: 1093-2607

DAY: 12

MONTH: 03

YEAR: 2007

Generation, characterization and biological activity of CCL2 (MCP-1/JE) and CCL12 (MCP-5) specific antibodies. Information

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LANGUAGE: eng

NlmUniqueID: 9711270

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Grant and Affiliation Information for Generation, characterization and biological activity of CCL2 (MCP-1/JE) and CCL12 (MCP-5) specific antibodies.

AFFILIATION: Department of Molecular Discovery Technologies, Discovery Research, Centocor R&D Inc., 145 King of Prussia Road, Radnor, PA 19087, USA.

Country: Netherlands

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MEDLINETA: Hum Antibodies

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