Gene trap disruption of the mouse heparan sulfate 6-O-endosulfatase gene, Sulf2.

Gene trap disruption of the mouse heparan sulfate 6-O-endosulfatase gene, Sulf2. Research Abstract Details 

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Gene trap disruption of the mouse heparan sulfate 6-O-endosulfatase gene, Sulf2. Abstract Text:

David H Lum,Jenille Tan,Steven D Rosen,Zena Werb,

Heparan sulfate (HS) chains are found in the extracellular matrix, covalently linked to core proteins collectively termed heparan sulfate proteoglycans (HSPGs). A wealth of data has demonstrated roles for HSPGs in the regulation of many cell surface signaling pathways that are crucial during development. Variations in the sulfation pattern along the HS chains influence their ability to interact with molecules such as growth factors, chemokines, morphogens, and adhesion molecules. Sulf1 and Sulf2 are members of a class of recently identified genes that encode heparan sulfate 6-O-endosulfatases (Sulf genes). The removal of 6-O-sulfate from HS via SULF activity influences the function of many factors, including Wnt, fibroblast growth factor, hepatocyte growth factor, heparin-binding epidermal growth factor, and bone morphogenetic protein. Given their possible developmental roles, we have examined Sulf gene expression during mouse embryogenesis. The two Sulf genes are expressed in a broad range of tissues throughout development with largely nonoverlapping expression patterns. Sulf2 transcripts are expressed in the lung, heart, placenta, and ribs. We generated a mouse line possessing a gene trap disruption of the Sulf2 gene. Mice homozygous for the Sulf2 gene trap allele are viable and fertile and have no major developmental defects on several genetic backgrounds. However, we observed strain-specific, nonpenetrant defects affecting viability, lung development, and growth in Sulf2 homozygous animals. These data suggest that Sulf2 may have roles in several tissues but that there is compensation by and/or redundancy with Sulf1.

Gene trap disruption of the mouse heparan sulfate 6-O-endosulfatase gene, Sulf2. Publishing Authors By Initials

DH Lum,J Tan,SD Rosen,Z Werb,

For similar enzymes and coenzymes: enzymes: transferases: sulfur group transferases: sulfotransferases research abstracts see: enzymes and coenzymes: enzymes: transferases: sulfur group transferases: sulfotransferases research

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Gene trap disruption of the mouse heparan sulfate 6-O-endosulfatase gene, Sulf2. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Molecular and cellular biology

VOLUME: 27

Page Numbers: 678-88

Journal Abbreviation: Mol. Cell. Biol.

ISSN: 0270-7306

DAY: 20

MONTH: 11

YEAR: 2006

Gene trap disruption of the mouse heparan sulfate 6-O-endosulfatase gene, Sulf2. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8109087

Gene trap disruption of the mouse heparan sulfate 6-O-endosulfatase gene, Sulf2. Keywords Mesh Terms:

KEYWORDS: Sulfotransferases

MESH TERMS: physiology

Chemical & Substance for Abstract: Gene trap disruption of the mouse heparan sulfate 6-O-endosulfatase gene, Sulf2. Information

Substance Name: Sulfatases

Registry Number: EC 3.1.6.-

Grant and Affiliation Information for Gene trap disruption of the mouse heparan sulfate 6-O-endosulfatase gene, Sulf2.

AFFILIATION: Department of Anatomy, HSW 1323, University of California-San Francisco, CA 94143-0452, USA.

Country: United States

AGENCY: United States NHLBI

GRANT: HL 075602

ACRONYM: HL

MEDLINETA: Mol Cell Biol

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