Gene therapy for new bone formation using adeno-associated viral bone morphogenetic protein-2 vectors.

Gene therapy for new bone formation using adeno-associated viral bone morphogenetic protein-2 vectors. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Gene therapy for new bone formation using adeno-associated viral bone morphogenetic protein-2 vectors. Abstract Text:

Y Chen,K D K Luk,K M C Cheung,R Xu,M C Lin,W W Lu,J C Y Leong,H-F Kung,

Previous reports have suggested that bone morphogenetic protein (BMP) gene therapy could be applied for in vivo bone regeneration. However, these studies were conducted either using immunodeficient animals because of immunogenicity of adenovirus vectors, or using ex vivo gene transfer technique, which is much more difficult to handle. Adeno-associated virus (AAV) is a replication-defective virus without any association with immunogenicity and human disease. This study was conducted to investigate whether orthotopic new bone formation could be induced by in vivo gene therapy using AAV-based BMP2 vectors. To test the feasibility of this approach, we constructed an AAV vector carrying human BMP2 gene. Mouse myoblast cells (C2C12) transduced with this vector could produce and secrete biologically active BMP2 protein and induce osteogenic activity, which was confirmed by ELISA and alkaline phosphatase activity assay. For in vivo study, AAV-BMP2 vectors were directly injected into the hindlimb muscle of immunocompetent Sprague-Dawley rats. Significant new bone under X-ray films could be detected as early as 3 weeks postinjection. The ossification tissue was further examined by histological and immunohistochemical analysis. This study is, to our knowledge, the first to establish the feasibility of AAV-based BMP2 gene therapy for endochondral ossification in immunocompetent animals.

Gene therapy for new bone formation using adeno-associated viral bone morphogenetic protein-2 vectors. Publishing Authors By Initials

Y Chen,KD Luk,KM Cheung,R Xu,MC Lin,WW Lu,JC Leong,HF Kung,

For similar peptides: intercellular signaling peptides and proteins: cytokines: transforming growth factor beta research abstracts see: peptides: intercellular signaling peptides and proteins: cytokines: transforming growth factor beta research

PUBMED ID PMID:

MEDLINE DATE:

Gene therapy for new bone formation using adeno-associated viral bone morphogenetic protein-2 vectors. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Gene therapy

VOLUME: 10

Page Numbers: 1345-53

Journal Abbreviation: Gene Ther.

ISSN: 0969-7128

DAY: 19

MONTH: Aug

YEAR: 2003

Gene therapy for new bone formation using adeno-associated viral bone morphogenetic protein-2 vectors. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9421525

Gene therapy for new bone formation using adeno-associated viral bone morphogenetic protein-2 vectors. Keywords Mesh Terms:

KEYWORDS: Transforming Growth Factor beta

MESH TERMS: methods

Chemical & Substance for Abstract: Gene therapy for new bone formation using adeno-associated viral bone morphogenetic protein-2 vectors. Information

Substance Name: bone morphogenetic protein 2

Registry Number: 0

Grant and Affiliation Information for Gene therapy for new bone formation using adeno-associated viral bone morphogenetic protein-2 vectors.

AFFILIATION: Department of Orthopaedic Surgery, Faculty of Medicine, The University of Hong Kong, Hong Kong.

Country: England

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Gene Ther

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Gene therapy for new bone formation using adeno-associated viral bone morphogenetic protein-2 vectors Related Publications

• (R)-N-Methyl-3-(3-(125)I-pyridin-2-yloxy)-3-phenylpropan-1-amine: a novel probe for norepinephrine transporters.
• 18F-labeled styrylpyridines as PET agents for amyloid plaque imaging.
• 2-(2'-((Dimethylamino)methyl)-4'-(fluoroalkoxy)-phenylthio)benzenamine Derivatives as Serotonin Transporter Imaging Agents.
• 39.5% conversion of low-power Q-switched Nd:YAG laser radiation to 266 nm by use of a resonant ring cavity.
• 5-Chloro-2-(2'-((dimethylamino)methyl)-4'-iodophenylthio)benzenamine: a new serotonin transporter ligand.
• A meta-analysis of the effects of Lactobacillus buchneri on the fermentation and aerobic stability of corn and grass and small-grain silages.
• A novel gallium bisaminothiolate complex as a myocardial perfusion imaging agent.
• C3A cell behaviors on micropatterned chitosan collagen gelatin membranes.
• CXCR4 inhibition synergizes with cytotoxic chemotherapy in gliomas.
• Characterization of optically resolved 9-fluoropropyl-dihydrotetrabenazine as a potential PET imaging agent targeting vesicular monoamine transporters.
• Combination of adeno-associated virus and adenovirus vectors expressing bone morphogenetic protein-2 produces enhanced osteogenic activity in immunocompetent rats.
• Effects of a mixture of lactic acid bacteria applied as a freeze-dried or fresh culture on the fermentation of alfalfa silage.
• Fluoro-pegylated (FPEG) imaging agents targeting Abeta aggregates.
• Fluoroalkyl derivatives of dihydrotetrabenazine as positron emission tomography imaging agents targeting vesicular monoamine transporters.
• Gene therapy for new bone formation using adeno-associated viral bone morphogenetic protein-2 vectors.
• Inappropriate activation of the androgen receptor by nonsteroids: involvement of the Src kinase pathway and its therapeutic implications.
• Kaposi's sarcoma-associated herpesvirus-encoded protein kinase and its interaction with K-bZIP.
• Lentiviral vectors mediate stable and efficient gene delivery into primary murine natural killer cells.
• Lipase activity during experimental epidermal carcinogenesis.
• Male germ cell-associated kinase, a male-specific kinase regulated by androgen, is a coactivator of androgen receptor in prostate cancer cells.
• Neuromuscular complications of thyrotoxicosis.
• New diphenylacetylenes as probes for positron emission tomographic imaging of amyloid plaques.
• Optical interference in nonlinear photonic crystals.
• Quick assembly of 1,4-diphenyltriazoles as probes targeting beta-amyloid aggregates in Alzheimer's disease.
• Radioiodinated aza-diphenylacetylenes as potential SPECT imaging agents for beta-amyloid plaque detection.
• Screening of active ingredients of herbal medicine for interaction with CYP450 3A4.
• Short communication: the effect of water temperature on the viability of silage inoculants.
• The effect of feeding a dry enzyme mixture with fibrolytic activity on the performance of lactating cows and digestibility of a diet for sheep.
• The effects of Lactobacillus buchneri 40788 and Pediococcus pentosaceus R1094 on the fermentation of corn silage.
• Yeast gain-of-function mutations reveal structure function relationships conserved among different subfamilies of transient receptor potential channels.