Gene profiling and signaling pathways of Candida albicans keratitis.

Gene profiling and signaling pathways of Candida albicans keratitis. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Gene profiling and signaling pathways of Candida albicans keratitis. Abstract Text:

PURPOSE: To compare the global gene expression patterns in uninfected and fungus-infected mouse corneas at the onset of Candida albicans keratitis. METHODS: Fungal keratitis was generated by scarifying the corneal epithelium of BALB/c mice followed by topical inoculation with Candida albicans. Corneal infection was allowed to progress for one day, and total RNA was then extracted from excised corneas. Microarray was performed to detect 45,102 murine genes and processed to identify genetic regulation of signaling pathways. Selected genes encoding interleukins (IL), chemokine ligands, and other cytokines were confirmed by quantitative real-time reverse transcriptase polymerase chain reaction (RT-PCR). RESULTS: Compared to mock-inoculated control eyes, genetic microarray analysis of Candida albicans keratitis showed that 3,977 genes (8.8%) changed at least twofold and 1,672 genes (3.7%) changed at least fourfold. Hierarchical clustering identified that upregulated genes affected immune and inflammatory responses, intercellular signaling, and cellular proliferation. Pathways having more than 20% of their genes significantly upregulated signaled leukocyte extravasation, increased interleukin production, and affected toll-like receptors. Upregulated transcript levels for IL-1beta and IL-6 were confirmed by real-time RT-PCR. CONCLUSIONS: Host gene expression during the initial stage of Candida albicans keratitis involves pathways contributing to acute inflammation mediated by interleukins and other signals of leukocyte recruitment. This murine study confirms the involvement of innate immunity in the cornea during the initiation of Candida albicans keratitis.

Gene profiling and signaling pathways of Candida albicans keratitis. Publishing Authors By Initials

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Gene profiling and signaling pathways of Candida albicans keratitis. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Molecular vision

VOLUME: 14

Page Numbers: 1792-8

Journal Abbreviation: Mol. Vis.

ISSN: 1090-0535

DAY: 26

MONTH: 09

YEAR: 2008

Gene profiling and signaling pathways of Candida albicans keratitis. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9605351

Gene profiling and signaling pathways of Candida albicans keratitis. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Gene profiling and signaling pathways of Candida albicans keratitis. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Gene profiling and signaling pathways of Candida albicans keratitis.

AFFILIATION: Sid W. Richardson Ocular Microbiology Laboratory, Cullen Eye Institute, Baylor College of Medicine, Houston, TX.

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Mol Vis

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Gene profiling and signaling pathways of Candida albicans keratitis Related Publications

• Candida albicans strain-dependent virulence and Rim13p-mediated filamentation in experimental keratomycosis.
• Capnocytophaga keratitis.
• Clinical characteristics and outcome of Candida keratitis.
• Corneal virulence of Candida albicans strains deficient in Tup1-regulated genes.
• Ecologic effects on eye banking.
• Expression of matrix metalloproteinases 2 and 9 in experimental corneal injury and fungal keratitis.
• Gene profiling and signaling pathways of Candida albicans keratitis.
• Prevalence and spectrum of bacterial co-infection during fungal keratitis.
• Quality assessment and microbiologic screening of donor corneas.
• Rapidly progressive cataract and iris atrophy during treatment of Acanthamoeba keratitis.
• The clinical diagnosis of microbial keratitis.
• Therapeutic interventions for herpes simplex virus epithelial keratitis.