Gene expression responses over 24 h to lengthening and shortening contractions in human muscle: major changes in CSRP3, MUSTN1, SIX1, and FBXO32.

Gene expression responses over 24 h to lengthening and shortening contractions in human muscle: major changes in CSRP3, MUSTN1, SIX1, and FBXO32. Research Abstract Details 

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Gene expression responses over 24 h to lengthening and shortening contractions in human muscle: major changes in CSRP3, MUSTN1, SIX1, and FBXO32. Abstract Text:

Matthew C Kostek,Yi-Wen Chen,Daniel J Cuthbertson,Rongye Shi,Mark J Fedele,Karyn A Esser,Michael J Rennie,

Resistance training using lengthening (eccentric) contractions induces greater increases in muscle size than shortening (concentric) contractions, but the underlying molecular mechanisms are not clear. Using temporal expression profiling, we compared changes in gene expression within 24 h of an acute bout of each type of contractions conducted simultaneously in the quadriceps of different legs. Five healthy young men performed shortening contractions with one leg while the contralateral leg performed lengthening contractions. Biopsies were taken from both legs before exercise and 3, 6, and 24 h afterwards, in the fed state. Expression profiling (n = 3) was performed using a custom-made Affymetrix MuscleChip containing probe sets of approximately 3,300 known genes and expressed sequence tags expressed in skeletal muscle. We identified 51 transcripts differentially regulated between the two exercise modes. Using unsupervised hierarchical clustering, we identified four distinct clusters, three of which corresponded to unique functional categories (protein synthesis, stress response/early growth, and sarcolemmal structure). Using quantitative RT-PCR (n = 5), we verified expression changes (lengthening/shortening) in SIX1 (3 h, -1.9-fold, P < 0.001), CSRP3 (6 h, 2.9-fold, P < 0.05), and MUSTN1 (24 h, 4.3-fold, P < 0.05). We examined whether FBXO32/atrogin-1/MAFbx, a known regulator of protein breakdown and of muscle atrophy was differentially expressed: the gene was downregulated after lengthening contractions (3 h, 2.7-fold, P < 0.05; 6 h, 3.3-fold, P < 0.05; 24 h, 2.3-fold, P < 0.05). The results suggested that lengthening and shortening contractions activated distinct molecular pathways as early as 3 h postexercise. The molecular differences might contribute to mechanisms underlying the physiological adaptations seen with training using the two modes of exercise.

Gene expression responses over 24 h to lengthening and shortening contractions in human muscle: major changes in CSRP3, MUSTN1, SIX1, and FBXO32. Publishing Authors By Initials

MC Kostek,YW Chen,DJ Cuthbertson,R Shi,MJ Fedele,KA Esser,MJ Rennie,

For similar natural sciences: time: time factors research abstracts see: natural sciences: time: time factors research

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Gene expression responses over 24 h to lengthening and shortening contractions in human muscle: major changes in CSRP3, MUSTN1, SIX1, and FBXO32. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Physiological genomics

VOLUME: 31

Page Numbers: 42-52

Journal Abbreviation: Physiol. Genomics

ISSN: 1531-2267

DAY: 22

MONTH: 05

YEAR: 2007

Gene expression responses over 24 h to lengthening and shortening contractions in human muscle: major changes in CSRP3, MUSTN1, SIX1, and FBXO32. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9815683

Gene expression responses over 24 h to lengthening and shortening contractions in human muscle: major changes in CSRP3, MUSTN1, SIX1, and FBXO32. Keywords Mesh Terms:

KEYWORDS: Time Factors

MESH TERMS: genetics

Chemical & Substance for Abstract: Gene expression responses over 24 h to lengthening and shortening contractions in human muscle: major changes in CSRP3, MUSTN1, SIX1, and FBXO32. Information

Substance Name: SKP Cullin F-Box Protein Ligases

Registry Number: EC 6.3.2.19

Grant and Affiliation Information for Gene expression responses over 24 h to lengthening and shortening contractions in human muscle: major changes in CSRP3, MUSTN1, SIX1, and FBXO32.

AFFILIATION: Center for Genetic Medicine Research, Children's National Medical Center, Washington, District of Columbia 20010, USA.

Country: United States

AGENCY: United Kingdom Wellcome T

GRANT: 49869

ACRONYM: HD

MEDLINETA: Physiol Genomics

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