Gene expression, fiber type, and strength are similar between left and right legs in older adults.

Gene expression, fiber type, and strength are similar between left and right legs in older adults. Research Abstract Details 

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Gene expression, fiber type, and strength are similar between left and right legs in older adults. Abstract Text:

Mark Tarnopolsky,Stuart Phillips,Gianni Parise,Alex Varbanov,James Demuth,Paul Stevens,Angela Qu,Feng Wang,Robert Isfort,Mark Tarnopolsky,Stuart Phillips,Gianni Parise,Alex Varbanov,James Demuth,Paul Stevens,Angela Qu,Feng Wang,Robert Isfort,

Methodological issues relevant to studies using microarrays and reverse transcription-polymerase chain reaction (RT-PCR) in human aging have rarely been evaluated. Because aging may accentuate biological differences between muscles, we compared transcriptome expression patterns, targeted messenger RNA (mRNA) abundance, strength, and muscle fiber type in the right and left legs of older adults. Muscle biopsies were taken from each Vastus lateralis in eight older (71 +/- 2 years) men, and isometric strength was determined. Samples were analyzed using an Affymetrix gene array, ATPase histochemistry, and RT-PCR for mRNA species involved in metabolism, apoptosis, vascular growth, and antioxidant status. Microarray analysis found that 31 of 5499 genes (0.6%) were significantly different between legs (negative log of the p value [NLOGP] >/= 2.0, but fold < 1.5), with only one gene, jumonji domain containing 1C (JMJD1C), being significantly different by >/= 1.50-fold. None of the mRNA species, or muscle fiber type, size, or strength, was different between legs. These findings are important for the design and analysis of studies using muscle data in older adults.

Gene expression, fiber type, and strength are similar between left and right legs in older adults. Publishing Authors By Initials

M Tarnopolsky,S Phillips,G Parise,A Varbanov,J Demuth,P Stevens,A Qu,F Wang,R Isfort,M Tarnopolsky,S Phillips,G Parise,A Varbanov,J Demuth,P Stevens,A Qu,F Wang,R Isfort,

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Gene expression, fiber type, and strength are similar between left and right legs in older adults. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: The journals of gerontology. Series A, Biological

VOLUME: 62

Page Numbers: 1088-95

Journal Abbreviation: J. Gerontol. A Biol. Sci. Med.

ISSN: 1079-5006

DAY: 20

MONTH: Oct

YEAR: 2007

Gene expression, fiber type, and strength are similar between left and right legs in older adults. Information

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LANGUAGE: eng

NlmUniqueID: 9502837

Gene expression, fiber type, and strength are similar between left and right legs in older adults. Keywords Mesh Terms:

KEYWORDS: Reverse Transcriptase Polymerase Chain R

MESH TERMS: genetics

Chemical & Substance for Abstract: Gene expression, fiber type, and strength are similar between left and right legs in older adults. Information

Substance Name: Oxidoreductases, N-Demethylating

Registry Number: EC 1.5.-

Grant and Affiliation Information for Gene expression, fiber type, and strength are similar between left and right legs in older adults.

AFFILIATION: McMaster University Medical Center, Pediatrics and Medicine, 1200 Main Street W, Hamilton, Ontario, Canada. tarnopol@mcmaster.ca

Country: United States

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MEDLINETA: J Gerontol A Biol Sci Med Sci

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