Gene expression changes associated with Barrett's esophagus and Barrett's-associated adenocarcinoma cell lines after acid or bile salt exposure.

Gene expression changes associated with Barrett's esophagus and Barrett's-associated adenocarcinoma cell lines after acid or bile salt exposure. Research Abstract Details 

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Gene expression changes associated with Barrett's esophagus and Barrett's-associated adenocarcinoma cell lines after acid or bile salt exposure. Abstract Text:

Ying Hao,Sumita Sood,George Triadafilopoulos,Jong Hyeok Kim,Zheng Wang,Peyman Sahbaie,M Bishr Omary,Anson W Lowe,

BACKGROUND: Esophageal reflux and Barrett's esophagus represent two major risk factors for the development of esophageal adenocarcinoma. Previous studies have shown that brief exposure of the Barrett's-associated adenocarcinoma cell line, SEG-1, or primary cultures of Barrett's esophageal tissues to acid or bile results in changes consistent with cell proliferation. In this study, we determined whether similar exposure to acid or bile salts results in gene expression changes that provide insights into malignant transformation. METHODS: Using previously published methods, Barrett's-associated esophageal adenocarcinoma cell lines and primary cultures of Barrett's esophageal tissue were exposed to short pulses of acid or bile salts followed by incubation in culture media at pH 7.4. A genome-wide assessment of gene expression was then determined for the samples using cDNA microarrays. Subsequent analysis evaluated for statistical differences in gene expression with and without treatment. RESULTS: The SEG-1 cell line showed changes in gene expression that was dependent on the length of exposure to pH 3.5. Further analysis using the Gene Ontology, however, showed that representation by genes associated with cell proliferation is not enhanced by acid exposure. The changes in gene expression also did not involve genes known to be differentially expressed in esophageal adenocarcinoma. Similar experiments using short-term primary cultures of Barrett's esophagus also did not result in detectable changes in gene expression with either acid or bile salt exposure. CONCLUSION: Short-term exposure of esophageal adenocarcinoma SEG-1 cells or primary cultures of Barrett's esophagus does not result in gene expression changes that are consistent with enhanced cell proliferation. Thus other model systems are needed that may reflect the impact of acid and bile salt exposure on the esophagus in vivo.

Gene expression changes associated with Barrett's esophagus and Barrett's-associated adenocarcinoma cell lines after acid or bile salt exposure. Publishing Authors By Initials

Y Hao,S Sood,G Triadafilopoulos,JH Kim,Z Wang,P Sahbaie,MB Omary,AW Lowe,

For similar investigative techniques: epidemiologic methods: statistics as topic: sensitivity and specificity research abstracts see: investigative techniques: epidemiologic methods: statistics as topic: sensitivity and specificity research

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Gene expression changes associated with Barrett's esophagus and Barrett's-associated adenocarcinoma cell lines after acid or bile salt exposure. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: BMC gastroenterology

VOLUME: 7

Page Numbers: 24

Journal Abbreviation:

ISSN: 1471-230X

DAY: 27

MONTH: 06

YEAR: 2007

Gene expression changes associated with Barrett's esophagus and Barrett's-associated adenocarcinoma cell lines after acid or bile salt exposure. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 100968547

Gene expression changes associated with Barrett's esophagus and Barrett's-associated adenocarcinoma cell lines after acid or bile salt exposure. Keywords Mesh Terms:

KEYWORDS: Sensitivity and Specificity

MESH TERMS: pathology

Chemical & Substance for Abstract: Gene expression changes associated with Barrett's esophagus and Barrett's-associated adenocarcinoma cell lines after acid or bile salt exposure. Information

Substance Name: DNA, Neoplasm

Registry Number: 0

Grant and Affiliation Information for Gene expression changes associated with Barrett's esophagus and Barrett's-associated adenocarcinoma cell lines after acid or bile salt exposure.

AFFILIATION: Department of Medicine, Stanford University, Stanford, CA, USA. yhao@stanford.edu <yhao@stanford.edu>

Country: England

AGENCY: United States NIDDK

GRANT: R01 DK063624

ACRONYM: DK

MEDLINETA: BMC Gastroenterol

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