Gene expression by fibroblasts seeded on small intestinal submucosa and subjected to cyclic stretching.

Gene expression by fibroblasts seeded on small intestinal submucosa and subjected to cyclic stretching. Research Abstract Details 

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Gene expression by fibroblasts seeded on small intestinal submucosa and subjected to cyclic stretching. Abstract Text:

Thomas W Gilbert,Ann M Stewart-Akers,Jennifer Sydeski,Tan D Nguyen,Stephen F Badylak,Savio L-Y Woo,

Extracellular matrix scaffolds derived from porcine small intestinal submucosa (SIS-ECM) have been shown to promote the formation of site-specific tissue in a number of preclinical animal studies. However, this constructive remodeling process requires that the scaffold be subjected to a site-specific mechanical environment. The specific quantitative effects of mechanical loading on the gene expression patterns of fibroblasts seeded on SIS-ECM are unknown and yet very important in the tissue remodeling process. The objective of the present study was to evaluate the expression of collagen type I (Col I), collagen type III (Col III), smooth muscle actin (SMA), tenascin-C (TN-C), matrix metalloprotease-2 (MMP-2), matrix metalloprotease-9 (MMP-9), transforming growth factor-beta1 (TGF-beta1), and transforming growth factor-beta3 (TGF-beta3) by fibroblasts subjected to various magnitudes (0%, 5%, 10%, and 15%) and frequencies (0.1 Hz, 0.3 Hz, and 0.5 Hz) of stretch. A new cyclic-stretching tissue culture (CSTC) system was developed. This system consists of eight independently controlled culture chambers that can be operated in a sterile incubator. Each chamber includes a load cell so that the load in each scaffold can be monitored. It was found that different stretching regimens led to complex and distinctive patterns of gene expression by fibroblasts seeded onto SIS-ECM. In general, the fibroblasts increased expression of Col I up to 5-fold and decreased that of Col III with increased frequency of stretch. In addition, the fibroblasts exhibited a contractile phenotype with increased expression of SMA, TN-C, and TGF-beta1. These findings support the concept that the mechanical environment of a remodeling ECM scaffold may have substantial effects on the behavior of cells within the scaffold and contribute to the site-specific tissue remodeling that has been observed in in vivo studies.

Gene expression by fibroblasts seeded on small intestinal submucosa and subjected to cyclic stretching. Publishing Authors By Initials

TW Gilbert,AM Stewart-Akers,J Sydeski,TD Nguyen,SF Badylak,SL Woo,

For similar investigative techniques: clinical laboratory techniques: culture techniques: tissue engineering research abstracts see: investigative techniques: clinical laboratory techniques: culture techniques: tissue engineering research

PUBMED ID PMID:

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Gene expression by fibroblasts seeded on small intestinal submucosa and subjected to cyclic stretching. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Tissue engineering

VOLUME: 13

Page Numbers: 1313-23

Journal Abbreviation: Tissue Eng.

ISSN: 1076-3279

DAY: 3

MONTH: Jun

YEAR: 2007

Gene expression by fibroblasts seeded on small intestinal submucosa and subjected to cyclic stretching. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9505538

Gene expression by fibroblasts seeded on small intestinal submucosa and subjected to cyclic stretching. Keywords Mesh Terms:

KEYWORDS: Tissue Engineering

MESH TERMS: methods

Chemical & Substance for Abstract: Gene expression by fibroblasts seeded on small intestinal submucosa and subjected to cyclic stretching. Information

Substance Name: Extracellular Matrix Proteins

Registry Number: 0

Grant and Affiliation Information for Gene expression by fibroblasts seeded on small intestinal submucosa and subjected to cyclic stretching.

AFFILIATION: McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, USA.

Country: United States

AGENCY: United States NIBIB

GRANT: EB000506-04

ACRONYM: EB

MEDLINETA: Tissue Eng

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