Gene expression analysis of zebrafish heart regeneration.

Gene expression analysis of zebrafish heart regeneration. Research Abstract Details 

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Gene expression analysis of zebrafish heart regeneration. Abstract Text:

Ching-Ling Lien,Michael Schebesta,Shinji Makino,Gerhard J Weber,Mark T Keating,

Mammalian hearts cannot regenerate. In contrast, zebrafish hearts regenerate even when up to 20% of the ventricle is amputated. The mechanism of zebrafish heart regeneration is not understood. To systematically characterize this process at the molecular level, we generated transcriptional profiles of zebrafish cardiac regeneration by microarray analyses. Distinct gene clusters were identified based on temporal expression patterns. Genes coding for wound response/inflammatory factors, secreted molecules, and matrix metalloproteinases are expressed in regenerating heart in sequential patterns. Comparisons of gene expression profiles between heart and fin regeneration revealed a set of regeneration core molecules as well as tissue-specific factors. The expression patterns of several secreted molecules around the wound suggest that they play important roles in heart regeneration. We found that both platelet-derived growth factor-a and -b (pdgf-a and pdgf-b) are upregulated in regenerating zebrafish hearts. PDGF-B homodimers induce DNA synthesis in adult zebrafish cardiomyocytes. In addition, we demonstrate that a chemical inhibitor of PDGF receptor decreases DNA synthesis of cardiomyocytes both in vitro and in vivo during regeneration. Our data indicate that zebrafish heart regeneration is associated with sequentially upregulated wound healing genes and growth factors and suggest that PDGF signaling is required.

Gene expression analysis of zebrafish heart regeneration. Publishing Authors By Initials

CL Lien,M Schebesta,S Makino,GJ Weber,MT Keating,

For similar proteins: fish proteins: zebrafish proteins research abstracts see: proteins: fish proteins: zebrafish proteins research

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Gene expression analysis of zebrafish heart regeneration. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: PLoS biology

VOLUME: 4

Page Numbers: e260

Journal Abbreviation: PLoS Biol.

ISSN: 1545-7885

DAY: 3

MONTH: Aug

YEAR: 2006

Gene expression analysis of zebrafish heart regeneration. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101183755

Gene expression analysis of zebrafish heart regeneration. Keywords Mesh Terms:

KEYWORDS: Zebrafish Proteins

MESH TERMS: metabolism

Chemical & Substance for Abstract: Gene expression analysis of zebrafish heart regeneration. Information

Substance Name: Matrix Metalloproteinases

Registry Number: EC 3.4.24.-

Grant and Affiliation Information for Gene expression analysis of zebrafish heart regeneration.

AFFILIATION: Department of Cardiology, Children's Hospital, Boston, Massachusetts, USA. lien@enders.tch.harvard.edu

Country: United States

AGENCY: United States NHLBI

GRANT: SCCOR RFA HL02–027

ACRONYM: HL

MEDLINETA: PLoS Biol

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