Gel-filtrated fractions of alveolar bone extract contain factors promoting cell attachment and a mitogenic effect on periodontal ligament fibroblasts.

Gel-filtrated fractions of alveolar bone extract contain factors promoting cell attachment and a mitogenic effect on periodontal ligament fibroblasts. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Gel-filtrated fractions of alveolar bone extract contain factors promoting cell attachment and a mitogenic effect on periodontal ligament fibroblasts. Abstract Text:

The purpose of this study was to investigate the effect of acetic acid-extracted bone proteins on human periodontal ligament fibroblasts (hPF) with respect to mitogenic and cell attachment promoting activity. Alveolar bone was harvested from healthy donors and subjected to 0.5 M acetic acid extraction, dialysis and lyophilization, and gel filtration. Promotion of cell attachment and stimulation of DNA synthesis by the crude extract and gel-filtrated fractions were studied in cultured hPE Many protein components, varying in molecular weight from 10-14 to 120 kDa, were detectable in 10% SDS-PAGE of the extract. Gel filtration of bone extract disclosed four fractions with molecular weights of 55, 34, 29 and 19-20 kDa. Both the 34 and 55 kDa fractions at a concentration of 5 microg/ml, but not the 29- or 19-20 kDa fractions, were found to promote cell attachment while only the 55 kDa fraction (5 microg/ml) stimulated DNA synthesis of hPF, Both mitogenic activity and the promotion of the cell attachment by gel-filtrated active fractions were resistant to thermal treatment (70 degrees C) and pH (4 to approximately 8) changes. These findings suggest that acetic acid extract of alveolar bone may contain components which are capable of modulating cell attachment and mitogenesis of hPF.

Gel-filtrated fractions of alveolar bone extract contain factors promoting cell attachment and a mitogenic effect on periodontal ligament fibroblasts. Publishing Authors By Initials

For similar complex mixtures: tissue extracts research abstracts see: complex mixtures: tissue extracts research

PUBMED ID PMID:

MEDLINE DATE:

Gel-filtrated fractions of alveolar bone extract contain factors promoting cell attachment and a mitogenic effect on periodontal ligament fibroblasts. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Proceedings of the National Science Council, Repub

VOLUME: 24

Page Numbers: 33-40

Journal Abbreviation: Proc. Natl. Sci. Counc. Repub.

ISSN: 0255-6596

DAY: 26

MONTH: Jan

YEAR: 2000

Gel-filtrated fractions of alveolar bone extract contain factors promoting cell attachment and a mitogenic effect on periodontal ligament fibroblasts. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8502426

Gel-filtrated fractions of alveolar bone extract contain factors promoting cell attachment and a mitogenic effect on periodontal ligament fibroblasts. Keywords Mesh Terms:

KEYWORDS: Tissue Extracts

MESH TERMS: pharmacology

Chemical & Substance for Abstract: Gel-filtrated fractions of alveolar bone extract contain factors promoting cell attachment and a mitogenic effect on periodontal ligament fibroblasts. Information

Substance Name: Acetic Acid

Registry Number: 64-19-7

Grant and Affiliation Information for Gel-filtrated fractions of alveolar bone extract contain factors promoting cell attachment and a mitogenic effect on periodontal ligament fibroblasts.

AFFILIATION: Department of Periodontology and Orthodontics, Graduate Institute of Clinical Dental Sciences, School of Dentistry, College of Medicine, National Taiwan University, Taipei, ROC.

Country: CHINA (REPUBLIC: 1949- )

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Proc Natl Sci Counc Repub Chin

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Gel-filtrated fractions of alveolar bone extract contain factors promoting cell attachment and a mitogenic effect on periodontal ligament fibroblasts Related Publications

• A new function of BMP4: dual role for BMP4 in regulation of Sonic hedgehog expression in the mouse tooth germ.
• An experimental study of anti-angiogenesis with recombinant human kringle 5.
• Application of Nd-Yag laser for gingivoplasty during orthodontic treatment.
• Clinical study of alveolar vertical distraction osteogenesis for implant.
• Enzyme-linked immunosorbent assay for the determination of p21-activated kinase activity.
• Hemorheology and expression of neutrophil adhesion molecules CD18 and CD62L in pancreatic microcirculation of Caerulein induced experimental acute pancreatitis.
• Lack of effect of dietary alpha-tocopherol on chemically induced hepatocarcinogenesis in rats.
• Prostaglandin E2 is involved in the increase of cytochrome P-450 2B1 expression by alpha-tocopheryl succinate in primary rat hepatocytes in the presence of phenobarbital.
• Rescue of cleft palate in Msx1-deficient mice by transgenic Bmp4 reveals a network of BMP and Shh signaling in the regulation of mammalian palatogenesis.
• Transgenically ectopic expression of Bmp4 to the Msx1 mutant dental mesenchyme restores downstream gene expression but represses Shh and Bmp2 in the enamel knot of wild type tooth germ.
• [A study on apoptosis and activity of SAPK/JNK in adrenocortical cells induced by cadmium chloride]
• [A study on central zone contour of tooth-crown vesticular surface among young people with normal occlusion]
• [Bone-inducing activity of human bone morphogenetic protein-2 102 peptide]
• [Comparative effects of tensiomin and sodium dimercaptosulphonate on the serum sulfydryl and anticopper of the patients with Wilson's disease]
• [Effects of fluoride on the expression of c-fos and c-jun genes and cell proliferation of rat osteoblasts]
• [Establishment of EBV-immortalized lymphoblast cell lines from three Chinese Fanconi anemia patient and their subtyping]
• [Experimental methods for mechanically stimulating the cells in vitro]
• [Focal adhesion kinase]
• [GATA-2 gene expression in leukemia patients and its significance]
• [Genetic polymorphisms of cytochrome P450 1A1 and susceptibility of early-onset Parkinson's disease]
• [Influence of chemotherapy on hematopoietic microenvironment and effect of autologous bone marrow stromal cell infusion on the recovery of hematopoiesis after chemotherapy]
• [Influence of the stratum corneum and dermis of the skin on the percutaneous absorption of fluorouracil delivered through skin patch]
• [Isolation, cultivation and identification of neural stem cell from human embryonic CNS]
• [Melanoma antigen-3 expression in human hepatocellular carcinoma]
• [Novel full-length cDNA cloning from normal adrenal gland and pheochromocytoma and functional prediction]
• [Preparation and application of a new chloromethylstyrene encapsulated silica single anion column]
• [Study on the intestinal microbial colonization resistance in patients with chronic severe hepatitis]
• [Synergetic transactivating functions of HCV core and truncated HBV middle surface protein]
• [Telomerase activity and cellular apoptosis of bladder cancers]
• [The apolipoprotein E gene polymorphism in children with steroid-resistant idiopathic nephrotic syndrome]