Gastrointestinal Distribution and Kinetic Characterization of the Sodium-Hydrogen Exchanger Isoform 8 (NHE8).

Gastrointestinal Distribution and Kinetic Characterization of the Sodium-Hydrogen Exchanger Isoform 8 (NHE8). Research Abstract Details 

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Gastrointestinal Distribution and Kinetic Characterization of the Sodium-Hydrogen Exchanger Isoform 8 (NHE8). Abstract Text:

Hua Xu,Huacong Chen,Jiali Dong,Ronald Lynch,Fayez K Ghishan,Hua Xu,Huacong Chen,Jiali Dong,Ronald Lynch,Fayez K Ghishan,Hua Xu,Huacong Chen,Jiali Dong,Ronald Lynch,Fayez K Ghishan,

NHE8 is a newly identified NHE isoform expressed in rat intestine. To date, the kinetic characteristics and the intestinal segmental distribution of this NHE isoform have not been studied. This current work was performed to determine the gene expression pattern of the NHE8 transporter along the gastrointestinal tract, as well as its affinity for Na(+), H(+), and sensitivity to known NHE inhibitors HOE694 and S3226. NHE8 was differentially expressed along the GI tract. Higher NHE8 expression was seen in stomach, duodenum, and ascending colon in human, while higher NHE8 expression was seen in jejunum, ileum and colon in adult mouse. Moreover, the expression level of NHE8 is much higher in the stomach and jejunum in young mice compared with adult mice. To evaluate the functional characterictics of NHE8, the pH indicator SNARF-4 was used to monitor the rate of intra-cellular pH (pH(i)) recovery after an NH(4)Cl induced acid load in NHE8 cDNA transfected PS120 cells. The NHE8 cDNA transfected cells exhibited a sodium-dependent proton exchanger activity having a Km for pH(i) of approximately pH 6.5, and a Km for sodium of approximately 23mM. Low concentration of HOE694 (1 muM) had no effect on NHE8 activity, while high concentration (10 muM) significantly reduced NHE8 activity. In the presence of 80 muM S3226, the NHE8 activity was also inhibited significantly. In conclusion, our work suggests that NHE8 is expressed along the gastrointestinal tract and NHE8 is a functional Na(+)/H(+) exchanger with kinetic characteristics that differ from other apically expressed NHE isoforms. Copyright (c) 2008 S. Karger AG, Basel.

Gastrointestinal Distribution and Kinetic Characterization of the Sodium-Hydrogen Exchanger Isoform 8 (NHE8). Publishing Authors By Initials

H Xu,H Chen,J Dong,R Lynch,FK Ghishan,H Xu,H Chen,J Dong,R Lynch,FK Ghishan,H Xu,H Chen,J Dong,R Lynch,FK Ghishan,

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Gastrointestinal Distribution and Kinetic Characterization of the Sodium-Hydrogen Exchanger Isoform 8 (NHE8). Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Cellular physiology and biochemistry : internation

VOLUME: 21

Page Numbers: 109-16

Journal Abbreviation: Cell. Physiol. Biochem.

ISSN: 1015-8987

DAY: 16

MONTH: 01

YEAR: 2008

Gastrointestinal Distribution and Kinetic Characterization of the Sodium-Hydrogen Exchanger Isoform 8 (NHE8). Information

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LANGUAGE: eng

NlmUniqueID: 9113221

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Grant and Affiliation Information for Gastrointestinal Distribution and Kinetic Characterization of the Sodium-Hydrogen Exchanger Isoform 8 (NHE8).

AFFILIATION: University of Arizona Health Sciences Center, Tucson, Arizona, USA.

Country: Switzerland

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MEDLINETA: Cell Physiol Biochem

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