Garlic compounds induced calpain and intrinsic caspase cascade for apoptosis in human malignant neuroblastoma SH-SY5Y cells.

Garlic compounds induced calpain and intrinsic caspase cascade for apoptosis in human malignant neuroblastoma SH-SY5Y cells. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Garlic compounds induced calpain and intrinsic caspase cascade for apoptosis in human malignant neuroblastoma SH-SY5Y cells. Abstract Text:

Surajit Karmakar,Naren L Banik,Sunil J Patel,Swapan K Ray,Surajit Karmakar,Naren L Banik,Sunil J Patel,Swapan K Ray,

Malignant (N-type) neuroblastoma continues to defy current chemotherapeutic regimens. We tested the garlic compounds diallyl sulfide (DAS) and diallyl disulfide (DADS) for induction of apoptosis in human malignant neuroblastoma SH-SY5Y cells. Viability of human primary neurons was unaffected after 24 h treatment with 50 and 100 microM DAS and 50 microM DADS but slightly affected with 100 microM DADS. Treatment with 50 and 100 microM DAS or DADS significantly decreased viability in SH-SY5Y cells. Wright staining showed morphological features of apoptosis in SH-SY5Y cells treated with 50 and 100 microM DAS or DADS for 24 h. ApopTag assay demonstrated DNA fragmentation in apoptotic cells. Apoptosis was associated with an increase in [Ca(2+)](i), increase in Bax:Bcl-2 ratio, mitochondrial release of cytochrome c, increase in cytosolic Smac/Diablo, and down regulation of inhibitor-of-apoptosis proteins and nuclear factor kappa B (NFkappaB). Activation of caspase-9 and caspase-3 indicated involvement of intrinsic pathway of apoptosis. Calpain and caspase-3 activities produced 145 kD spectrin break down product (SBDP) and 120 kD SBDP, respectively. Also, caspase-3 activity cleaved inhibitor of caspase-activated DNase (ICAD). Results strongly suggested that the garlic compounds DAS and DADS suppressed anti-apoptotic factors and activated calpain and intrinsic caspase cascade for apoptosis in SH-SY5Y cells.

Garlic compounds induced calpain and intrinsic caspase cascade for apoptosis in human malignant neuroblastoma SH-SY5Y cells. Publishing Authors By Initials

S Karmakar,NL Banik,SJ Patel,SK Ray,S Karmakar,NL Banik,SJ Patel,SK Ray,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Garlic compounds induced calpain and intrinsic caspase cascade for apoptosis in human malignant neuroblastoma SH-SY5Y cells. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Apoptosis : an international journal on programmed

VOLUME: 12

Page Numbers: 671-84

Journal Abbreviation: Apoptosis

ISSN: 1360-8185

DAY: 12

MONTH: Apr

YEAR: 2007

Garlic compounds induced calpain and intrinsic caspase cascade for apoptosis in human malignant neuroblastoma SH-SY5Y cells. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9712129

Garlic compounds induced calpain and intrinsic caspase cascade for apoptosis in human malignant neuroblastoma SH-SY5Y cells. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Garlic compounds induced calpain and intrinsic caspase cascade for apoptosis in human malignant neuroblastoma SH-SY5Y cells. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Garlic compounds induced calpain and intrinsic caspase cascade for apoptosis in human malignant neuroblastoma SH-SY5Y cells.

AFFILIATION: Department of Neurosciences, Medical University of South Carolina, 96 Jonathan Lucas Street, Suite 323K, PO Box 250606, Charleston, SC 29425, USA.

Country: United States

AGENCY: United States NINDS

GRANT: R01 NS-57811

ACRONYM: NS

MEDLINETA: Apoptosis

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Garlic compounds induced calpain and intrinsic caspase cascade for apoptosis in human malignant neuroblastoma SH-SY5Y cells Related Publications

• 5-Aminolevulinic acid-based photodynamic therapy suppressed survival factors and activated proteases for apoptosis in human glioblastoma U87MG cells.
• A novel role of fibroblast growth factor-2 and pentosan polysulfate in the pathogenesis of intestinal bleeding in mice.
• Adenovirus-mediated gene transfer to renal glomeruli in rodents.
• Aldosterone impairs baroreflex sensitivity in healthy adults.
• Alkaloids of Fumaria indica: Further Studies on Narceimine and Narlumidine.
• Chemical Constituents of the Leaves of Flueggea microcarpa.
• Currently evaluated calpain and caspase inhibitors for neuroprotection in experimental brain ischemia.
• Deficient SOCS3 expression in CD4+CD25+FoxP3+ regulatory T cells and SOCS3-mediated suppression of Treg function.
• Effect of acute hyperlipidemia on autonomic and cardiovascular control in humans.
• Effect of elevating serum lipids on luteinizing hormone response to gonadotrophin releasing hormone challenge in energy-deficient anestrous heifers.
• Effects of Exercise TVaining on Brachial and Femoral Myogenic Responses in Young Subjects: 1685: Board #175 May 30 2:00 PM - 3:30 PM.
• Estrous response of early postpartum beef heifers to progesterone and estradiol-17beta during restricted dietary energy.
• Fallen between the Cracks: Conservation Linking Land and Sea.
• Garlic compounds induced calpain and intrinsic caspase cascade for apoptosis in human malignant neuroblastoma SH-SY5Y cells.
• Homozygous mutation of AURKC yields large-headed polyploid spermatozoa and causes male infertility.
• Increasing use of antidepressants in pregnancy.
• Induction of apoptosis and immune response by all-trans retinoic acid plus interferon-gamma in human malignant glioblastoma T98G and U87MG cells.
• Influence of static magnetic fields on pain perception and sympathetic nerve activity in humans.
• Major Alkaloid and Flavonoid of Premna integrifolia.
• Percent positive biopsy cores as a prognostic factor for prostate cancer treated with external beam radiation.
• Phenolic Constituents of Rhus semialata Leaves.
• Physalindicanols, New Biogenetic Precursors of C28-Steroidal Lactones from Physalis minima var. indica.
• Progression of fibrosis during chronic hepatitis C is associated with rapid virus evolution.
• RBx 7,796: A novel inhibitor of 5-lipoxygenase.
• Regulation and characterization of rot transcription in Staphylococcus aureus.
• Review of kinetics of chemical and photocatalytical oxidation of Arsenic(III) as influenced by pH.
• Small interfering RNA targeted to hepatitis C virus 5' nontranslated region exerts potent antiviral effect.
• Stellate cell apoptosis by a soluble mediator from immortalized human hepatocytes.
• Transfusion support to Dengue patients in a hospital based blood transfusion service in north India.
• Vascular endothelial growth factor expression in arthritic joint is regulated by SAF-1 transcription factor.