Gapex-5, a Rab31 guanine nucleotide exchange factor that regulates Glut4 trafficking in adipocytes.

Gapex-5, a Rab31 guanine nucleotide exchange factor that regulates Glut4 trafficking in adipocytes. Research Abstract Details 

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Gapex-5, a Rab31 guanine nucleotide exchange factor that regulates Glut4 trafficking in adipocytes. Abstract Text:

Irfan J Lodhi,Shian-Huey Chiang,Louise Chang,Daniel Vollenweider,Robert T Watson,Mayumi Inoue,Jeffrey E Pessin,Alan R Saltiel,

Insulin stimulates glucose uptake by promoting translocation of the Glut4 glucose transporter from intracellular storage compartments to the plasma membrane. In the absence of insulin, Glut4 is retained intracellularly; the mechanism underlying this process remains uncertain. Using the TC10-interacting protein CIP4 as bait in a yeast two-hybrid screen, we cloned a RasGAP and VPS9 domain-containing protein, Gapex-5/RME-6. The VPS9 domain is a guanine nucleotide exchange factor for Rab31, a Rab5 subfamily GTPase implicated in trans-Golgi network (TGN)-to-endosome trafficking. Overexpression of Rab31 blocks insulin-stimulated Glut4 translocation, whereas knockdown of Rab31 potentiates insulin-stimulated Glut4 translocation and glucose uptake. Gapex-5 is predominantly cytosolic in untreated cells; its overexpression promotes intracellular retention of Glut4 in adipocytes. Insulin recruits the CIP4/Gapex-5 complex to the plasma membrane, thus reducing Rab31 activity and permitting Glut4 vesicles to translocate to the cell surface, where Glut4 docks and fuses to transport glucose into the cell.

Gapex-5, a Rab31 guanine nucleotide exchange factor that regulates Glut4 trafficking in adipocytes. Publishing Authors By Initials

IJ Lodhi,SH Chiang,L Chang,D Vollenweider,RT Watson,M Inoue,JE Pessin,AR Saltiel,

For similar enzymes and coenzymes: enzymes: hydrolases: acid anhydride hydrolases: gtp phosphohydrolases: gtp-binding proteins: monomeric gtp-binding proteins: rab gtp-binding proteins: rab5 gtp-binding proteins research abstracts see: enzymes and coenzymes: enzymes: hydrolases: acid anhydride hydrolases: gtp phosphohydrolases: gtp-binding proteins: monomeric gtp-binding proteins: rab gtp-binding proteins: rab5 gtp-binding proteins research

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Gapex-5, a Rab31 guanine nucleotide exchange factor that regulates Glut4 trafficking in adipocytes. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Cell metabolism

VOLUME: 5

Page Numbers: 59-72

Journal Abbreviation: Cell Metab.

ISSN: 1550-4131

DAY: 3

MONTH: Jan

YEAR: 2007

Gapex-5, a Rab31 guanine nucleotide exchange factor that regulates Glut4 trafficking in adipocytes. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101233170

Gapex-5, a Rab31 guanine nucleotide exchange factor that regulates Glut4 trafficking in adipocytes. Keywords Mesh Terms:

KEYWORDS: rab5 GTP-Binding Proteins

MESH TERMS: metabolism

Chemical & Substance for Abstract: Gapex-5, a Rab31 guanine nucleotide exchange factor that regulates Glut4 trafficking in adipocytes. Information

Substance Name: rab5 GTP-Binding Proteins

Registry Number: EC 3.6.5.2

Grant and Affiliation Information for Gapex-5, a Rab31 guanine nucleotide exchange factor that regulates Glut4 trafficking in adipocytes.

AFFILIATION: Life Sciences Institute, University of Michigan, Ann Arbor, MI 48109, USA.

Country: United States

AGENCY: United States NIDDK

GRANT: R01-DK061618

ACRONYM: DK

MEDLINETA: Cell Metab

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