gammadelta T lymphocytes: a new type of regulatory T cells suppressing murine 2,4,6-trinitrobenzene sulphonic acid (TNBS)-induced colitis.

gammadelta T lymphocytes: a new type of regulatory T cells suppressing murine 2,4,6-trinitrobenzene sulphonic acid (TNBS)-induced colitis. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

gammadelta T lymphocytes: a new type of regulatory T cells suppressing murine 2,4,6-trinitrobenzene sulphonic acid (TNBS)-induced colitis. Abstract Text:

BACKGROUND: The intestinal immune system is continuously challenged by antigen without becoming dysregulated. However, injury of the mucosa by, i.e. dextran sulphate sodium causes severe inflammation in gammadelta T-cell-deficient mice. We therefore asked whether gammadelta T cells have regulatory functions. MATERIALS AND METHODS: gammadelta T cells were isolated from spleens and mesenteric lymph nodes of C57BL/6 wild-type (wt) mice. Proliferation and cytokine secretion of gammadelta T cells were quantified by [(3)H] thymidine incorporation and ELISA. Additionally, proliferation of carboxyfluorescein diacetate succinimidylester-labelled CD4(+) T cells cocultured with gammadelta T cells was analysed by flow cytometry. Finally, gammadelta T cells from wt or interleukin-10 transgenic (IL-10tg) mice were transferred into congenic mice with 2,4,6-trinitrobenzene sulphonic acid (TNBS)-induced colitis. RESULTS: gammadelta T cells were hyporesponsive to CD3/CD28 stimulation and suppressed CD4(+) T-cell proliferation (up to 66 +/- 7% suppression) in vitro. Further, the preventive transfer of wt or IL-10tg gammadelta T cells ameliorated TNBS-induced colitis resulting in prolonged survival and reduced histological damage (1.5 +/- 0.4 and 1.3 +/- 0.2, respectively vs. 3.8 +/- 0.3 in untransferred mice, p < 0.05). This was accompanied by reduced TNF-alpha and increased IL-10 and TGF-beta secretion from intestinal and splenic lymphocytes. CONCLUSIONS: Murine gammadelta T cells are a new type of regulatory T cells in vitro and act protective on mouse TNBS-induced colitis in vivo. Future studies have to define the underlying mechanism and to investigate whether gammadelta T cells can be used for immunotherapy of human inflammatory bowel disease.

gammadelta T lymphocytes: a new type of regulatory T cells suppressing murine 2,4,6-trinitrobenzene sulphonic acid (TNBS)-induced colitis. Publishing Authors By Initials

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

gammadelta T lymphocytes: a new type of regulatory T cells suppressing murine 2,4,6-trinitrobenzene sulphonic acid (TNBS)-induced colitis. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: International journal of colorectal disease

VOLUME: 23

Page Numbers: 909-20

Journal Abbreviation:

ISSN: 0179-1958

DAY: 23

MONTH: 07

YEAR: 2008

gammadelta T lymphocytes: a new type of regulatory T cells suppressing murine 2,4,6-trinitrobenzene sulphonic acid (TNBS)-induced colitis. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8607899

gammadelta T lymphocytes: a new type of regulatory T cells suppressing murine 2,4,6-trinitrobenzene sulphonic acid (TNBS)-induced colitis. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: gammadelta T lymphocytes: a new type of regulatory T cells suppressing murine 2,4,6-trinitrobenzene sulphonic acid (TNBS)-induced colitis. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for gammadelta T lymphocytes: a new type of regulatory T cells suppressing murine 2,4,6-trinitrobenzene sulphonic acid (TNBS)-induced colitis.

AFFILIATION: Medizinische Klinik I, Charité-Universitätsmedizin Berlin, Campus Benjamin Franklin, Hindenburgdamm 30, 12200, Berlin, Germany, joerg.hoffmann@st-marienkrankenhaus.de.

Country: Germany

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Int J Colorectal Dis

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

gammadelta T lymphocytes: a new type of regulatory T cells suppressing murine 2,4,6-trinitrobenzene sulphonic acid TNBS-induced colitis Related Publications

• Aortic valve calcification as a marker for aortic stenosis severity: assessment on 16-MDCT.
• Arteriovenous angioma of the vein of Galen causing cardiac failure in the neonate. Report on clinical and pathological findings in two cases.
• Contraception in diabetic women.
• DM-GRASP/ALCAM/CD166 is required for cardiac morphogenesis and maintenance of cardiac identity in first heart field derived cells.
• From individual Wnt pathways towards a Wnt signalling network.
• Gastrointestinal hormones and cortisol in normal pregnant women and women with gestational diabetes.
• Giant-cell myocarditis in a patient presenting with dilated cardiomyopathy and ventricular tachycardias treated by immunosuppression: A case report.
• Metabolic adaptations during the neonatal period in infants of diabetic mothers.
• Pescadillo is required for Xenopus laevis eye development and neural crest migration.
• Regulation of nif gene expression and the energetics of N(2) fixation over the diel cycle in a hot spring microbial mat.
• Repulsive guidance molecule A (RGM A) and its receptor neogenin during neural and neural crest cell development of Xenopus laevis.
• Suspected Chronic Myocarditis at Cardiac MR: Diagnostic Accuracy and Association with Immunohistologically Detected Inflammation and Viral Persistence.
• The NovoPen--a practical tool for simplifying multiple injection insulin therapy.
• [Can the high percentage of premature twins be reduced? Thoughts based on an analysis of 243 twin births]
• [State of diagnostics and therapy of inflammatory cardiomyopathie.]
• [Stress deformations of skeletal remains from prehistoric cremation burials]
• [Therapy of depressive states with hypericin]
• beta-Catenin signaling contributes to stemness and regulates early differentiation in murine embryonic stem cells.
• gammadelta T lymphocytes: a new type of regulatory T cells suppressing murine 2,4,6-trinitrobenzene sulphonic acid (TNBS)-induced colitis.