Galphai generates multiple Pins activation states to link cortical polarity and spindle orientation in Drosophila neuroblasts.

Galphai generates multiple Pins activation states to link cortical polarity and spindle orientation in Drosophila neuroblasts. Research Abstract Details 

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Galphai generates multiple Pins activation states to link cortical polarity and spindle orientation in Drosophila neuroblasts. Abstract Text:

Rick W Nipper,Karsten H Siller,Nicholas R Smith,Chris Q Doe,Kenneth E Prehoda,

Drosophila neuroblasts divide asymmetrically by aligning their mitotic spindle with cortical cell polarity to generate distinct sibling cell types. Neuroblasts asymmetrically localize Galphai, Pins, and Mud proteins; Pins/Galphai direct cortical polarity, whereas Mud is required for spindle orientation. It is currently unknown how Galphai-Pins-Mud binding is regulated to link cortical polarity with spindle orientation. Here, we show that Pins forms a "closed" state via intramolecular GoLoco-tetratricopeptide repeat (TPR) interactions, which regulate Mud binding. Biochemical, genetic, and live imaging experiments show that Galphai binds to the first of three Pins GoLoco motifs to recruit Pins to the apical cortex without "opening" Pins or recruiting Mud. However, Galphai and Mud bind cooperatively to the Pins GoLocos 2/3 and tetratricopeptide repeat domains, respectively, thereby restricting Pins-Mud interaction to the apical cortex and fixing spindle orientation. We conclude that Pins has multiple activity states that generate cortical polarity and link it with mitotic spindle orientation.

Galphai generates multiple Pins activation states to link cortical polarity and spindle orientation in Drosophila neuroblasts. Publishing Authors By Initials

RW Nipper,KH Siller,NR Smith,CQ Doe,KE Prehoda,

For similar biochemical phenomena, metabolism, and nutrition: biochemical phenomena: protein binding research abstracts see: biochemical phenomena, metabolism, and nutrition: biochemical phenomena: protein binding research

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Galphai generates multiple Pins activation states to link cortical polarity and spindle orientation in Drosophila neuroblasts. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Proceedings of the National Academy of Sciences of

VOLUME: 104

Page Numbers: 14306-11

Journal Abbreviation: Proc. Natl. Acad. Sci. U.S.A.

ISSN: 0027-8424

DAY: 28

MONTH: 08

YEAR: 2007

Galphai generates multiple Pins activation states to link cortical polarity and spindle orientation in Drosophila neuroblasts. Information

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LANGUAGE: eng

NlmUniqueID: 7505876

Galphai generates multiple Pins activation states to link cortical polarity and spindle orientation in Drosophila neuroblasts. Keywords Mesh Terms:

KEYWORDS: Protein Binding

MESH TERMS: metabolism

Chemical & Substance for Abstract: Galphai generates multiple Pins activation states to link cortical polarity and spindle orientation in Drosophila neuroblasts. Information

Substance Name: GTP-Binding Protein alpha Subunits, Gi-G

Registry Number: EC 3.6.1.46

Grant and Affiliation Information for Galphai generates multiple Pins activation states to link cortical polarity and spindle orientation in Drosophila neuroblasts.

AFFILIATION: Institute of Molecular Biology, Department of Chemistry, University of Oregon, Eugene, OR 97403, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: R01 GM068032-05

ACRONYM: GM

MEDLINETA: Proc Natl Acad Sci U S A

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