Galectin-1 in secondary alveolar septae of neonatal mouse lung.

Galectin-1 in secondary alveolar septae of neonatal mouse lung. Research Abstract Details 

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Galectin-1 in secondary alveolar septae of neonatal mouse lung. Abstract Text:

Jennifer J Foster,Kelli L Goss,Caroline L S George,Peter J Bangsund,Jeanne M Snyder,

In mice, alveolarization occurs during postnatal days 4 through 12, when secondary alveolar septae create thin-walled alveoli in the distal lung. We hypothesized that genes predominantly expressed in newly forming secondary alveolar septae influence the process of alveolarization. To address this hypothesis, tips of secondary alveolar septae were isolated from sections of postnatal day 6 mouse lung tissue using laser capture microdissection. Total RNA was isolated and amplified from the dissected alveolar septal tips and from intact postnatal day 6 lung tissue. Gene expression in the samples was characterized using Affymetrix mouse U74AN2 GeneChips. Galectin-1 was an abundantly expressed transcript that was enriched in the alveolar septal tips compared with levels in the whole lung tissue. Galectins are beta-galactoside-binding proteins involved in the regulation of cell proliferation, differentiation, and apoptosis in fibroblasts, muscle cells and endothelial cells, cell types that are present in the alveolar wall. Immunostaining in postnatal day 6 lung tissue confirmed that galectin-1 protein is concentrated in the tips of secondary alveolar septae, predominantly in myofibroblasts. Fibroblasts isolated from day 6 neonatal mouse lung tissue contained galectin-1 protein. Real-time PCR demonstrated that galectin-1 mRNA levels in mouse lung tissue peak at postnatal day 6. Immunoblot analysis confirmed that peak levels of lung galectin-1 protein are found at postnatal days 6 to 12. The increased expression of galectin-1 at the site and time of ongoing alveolarization in the newborn mouse is suggestive that galectin-1 may play an important role in this critical aspect of lung development.

Galectin-1 in secondary alveolar septae of neonatal mouse lung. Publishing Authors By Initials

JJ Foster,KL Goss,CL George,PJ Bangsund,JM Snyder,

For similar nucleic acids, nucleotides, and nucleosides: nucleic acids: rna: rna, messenger research abstracts see: nucleic acids, nucleotides, and nucleosides: nucleic acids: rna: rna, messenger research

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Galectin-1 in secondary alveolar septae of neonatal mouse lung. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: American journal of physiology. Lung cellular and

VOLUME: 291

Page Numbers: L1142-9

Journal Abbreviation: Am. J. Physiol. Lung Cell Mol.

ISSN: 1040-0605

DAY: 4

MONTH: 08

YEAR: 2006

Galectin-1 in secondary alveolar septae of neonatal mouse lung. Information

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LANGUAGE: eng

NlmUniqueID: 100901229

Galectin-1 in secondary alveolar septae of neonatal mouse lung. Keywords Mesh Terms:

KEYWORDS: RNA, Messenger

MESH TERMS: genetics

Chemical & Substance for Abstract: Galectin-1 in secondary alveolar septae of neonatal mouse lung. Information

Substance Name: RNA, Messenger

Registry Number: 0

Grant and Affiliation Information for Galectin-1 in secondary alveolar septae of neonatal mouse lung.

AFFILIATION: Department of Pediatrics, University of Iowa, Iowa City, Iowa, USA.

Country: United States

AGENCY: United States NHLBI

GRANT: T32-HL-07638

ACRONYM: HL

MEDLINETA: Am J Physiol Lung Cell Mol Phy

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