Galectin-1 binds different CD43 glycoforms to cluster CD43 and regulate T cell death.

Galectin-1 binds different CD43 glycoforms to cluster CD43 and regulate T cell death. Research Abstract Details 

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Galectin-1 binds different CD43 glycoforms to cluster CD43 and regulate T cell death. Abstract Text:

Joseph D Hernandez,Julie T Nguyen,Jiale He,Wei Wang,Blair Ardman,Jonathan M Green,Minoru Fukuda,Linda G Baum,

Galectin-1 kills immature thymocytes and activated peripheral T cells by binding to glycans on T cell glycoproteins including CD7, CD45, and CD43. Although roles for CD7 and CD45 in regulating galectin-1-induced death have been described, the requirement for CD43 remains unknown. We describe a novel role for CD43 in galectin-1-induced death, and the effects of O-glycan modification on galectin-1 binding to CD43. Loss of CD43 expression reduced galectin-1 death of murine thymocytes and human T lymphoblastoid cells, indicating that CD43 is required for maximal T cell susceptibility to galectin-1. CD43, which is heavily O-glycosylated, contributes a significant fraction of galectin-1 binding sites on T cells, as T cells lacking CD43 bound approximately 50% less galectin-1 than T cells expressing CD43. Although core 2 modification of O-glycans on other glycoprotein receptors is critical for galectin-1-induced cross-linking and T cell death, galectin-1 bound to CD43 fusion proteins modified with either unbranched core 1 or branched core 2 O-glycans and expression of core 2 O-glycans did not enhance galectin-1 binding to CD43 on T cells. Moreover, galectin-1 binding clustered CD43 modified with either core 1 or core 2 O-glycans on the T cell surface. Thus, CD43 bearing either core 1 or core 2 O-glycans can positively regulate T cell susceptibility to galectin-1, identifying a novel function for CD43 in controlling cell death. In addition, these studies demonstrate that different T cell glycoproteins on the same cell have distinct requirements for glycan modifications that allow recognition and cross-linking by galectin-1.

Galectin-1 binds different CD43 glycoforms to cluster CD43 and regulate T cell death. Publishing Authors By Initials

JD Hernandez,JT Nguyen,J He,W Wang,B Ardman,JM Green,M Fukuda,LG Baum,

For similar tissues: lymphoid tissue: thymus gland research abstracts see: tissues: lymphoid tissue: thymus gland research

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Galectin-1 binds different CD43 glycoforms to cluster CD43 and regulate T cell death. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of immunology (Baltimore, Md. : 1950)

VOLUME: 177

Page Numbers: 5328-36

Journal Abbreviation: J. Immunol.

ISSN: 0022-1767

DAY: 15

MONTH: Oct

YEAR: 2006

Galectin-1 binds different CD43 glycoforms to cluster CD43 and regulate T cell death. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985117

Galectin-1 binds different CD43 glycoforms to cluster CD43 and regulate T cell death. Keywords Mesh Terms:

KEYWORDS: Thymus Gland

MESH TERMS: cytology

Chemical & Substance for Abstract: Galectin-1 binds different CD43 glycoforms to cluster CD43 and regulate T cell death. Information

Substance Name: Polysaccharides

Registry Number: 0

Grant and Affiliation Information for Galectin-1 binds different CD43 glycoforms to cluster CD43 and regulate T cell death.

AFFILIATION: Department of Pathology and Laboratory Medicine, University of California Los Angeles School of Medicine, Los Angeles, CA 90095, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: T32 GM08042

ACRONYM: GM

MEDLINETA: J Immunol

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