Gadd45beta forms a Homodimeric Complex that Binds Tightly to MKK7.

Gadd45beta forms a Homodimeric Complex that Binds Tightly to MKK7. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Gadd45beta forms a Homodimeric Complex that Binds Tightly to MKK7. Abstract Text:

Laura Tornatore,Daniela Marasco,Nina Dathan,Rosa Maria Vitale,Ettore Benedetti,Salvatore Papa,Guido Franzoso,Menotti Ruvo,Simona Maria Monti,Laura Tornatore,Daniela Marasco,Nina Dathan,Rosa Maria Vitale,Ettore Benedetti,Salvatore Papa,Guido Franzoso,Menotti Ruvo,Simona Maria Monti,

Gadd45alpha, beta, and gamma proteins, also known as growth arrest and DNA damage-inducible factors, have a number of cellular functions, including cell-cycle regulation and propagation of signals produced by a variety of cellular stimuli, maintaining genomic stability and apoptosis. Furthermore, Gadd45beta has been indicated as a major player in the endogenous NF-kappaB-mediated resistance to apoptosis in a variety of cell lines. In fibroblasts this mechanism involves the inactivation of MKK7, the upstream activator of JNK, by direct binding within the kinase ATP pocket. On the basis of a number of experimental data, the structures of Gadd45beta and the Gadd45beta-MKK7 complex have been predicted recently and data show that interactions are mediated by acidic loops 1 and 2, and helices 3 and 4 of Gadd45beta. Here, we provide further evidence that Gadd45beta is a prevailingly alpha-helical protein and that in solution it is able to form non covalent dimers but not higher-order oligomers, in contrast to what has been reported for the homologous Gadd45alpha. We show that the contact region between the two monomers is comprised of the predicted helix 1 (residues Q17-Q33) and helix 5 (residues K131-R146) of the protein, which appear to be antiparallel and to form a large dimerisation surface not involved in MKK7 recognition. The results suggest the occurrence of a large complex containing at least an MKK7-Gadd45beta:Gadd45beta-MKK7 tetrameric unit whose complexity could be further increased by the dimeric nature of the isolated MKK7.

Gadd45beta forms a Homodimeric Complex that Binds Tightly to MKK7. Publishing Authors By Initials

L Tornatore,D Marasco,N Dathan,RM Vitale,E Benedetti,S Papa,G Franzoso,M Ruvo,SM Monti,L Tornatore,D Marasco,N Dathan,RM Vitale,E Benedetti,S Papa,G Franzoso,M Ruvo,SM Monti,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Gadd45beta forms a Homodimeric Complex that Binds Tightly to MKK7. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of molecular biology

VOLUME: 378

Page Numbers: 97-111

Journal Abbreviation:

ISSN: 1089-8638

DAY: 4

MONTH: 02

YEAR: 2008

Gadd45beta forms a Homodimeric Complex that Binds Tightly to MKK7. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985088

Gadd45beta forms a Homodimeric Complex that Binds Tightly to MKK7. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Gadd45beta forms a Homodimeric Complex that Binds Tightly to MKK7. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Gadd45beta forms a Homodimeric Complex that Binds Tightly to MKK7.

AFFILIATION: Istituto di Biostrutture e Bioimmagini (IBB), CNR, via Mezzocannone, 16, 80134, Napoli, Italy; Dipartimento delle Scienze Biologiche, via Mezzocannone, 16, 80134, Napoli, Italy.

Country: England

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Mol Biol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Gadd45beta forms a Homodimeric Complex that Binds Tightly to MKK7 Related Publications

• "I'll never drink like that again": characteristics of alcohol-related incidents and predictors of motivation to change in college students.
• Associations of the dopamine D4 receptor gene VNTR polymorphism with drug use in adolescent psychiatric inpatients.
• Causes of death in symptomatic cryoglobulinemia: 30 years of observation in a Department of Internal Medicine.
• Characterization of Static Disorder by Cumulant Analysis of EXAFS: an Investigation on a Two-Gaussian Distribution.
• Cocaine craving as a predictor of treatment attrition and outcomes after residential treatment for cocaine dependence.
• Correlates of motivation to quit smoking among alcohol dependent patients in residential treatment.
• Efficacy of counselor vs. computer-delivered intervention with mandated college students.
• Family history and antisocial traits moderate naltrexone's effects on heavy drinking in alcoholics.
• Inhibition of Stat3 increases doxorubicin sensitivity in a human metastatic breast cancer cell line.
• Involving families who have deaf children using a Family Needs Survey: a multi-agency perspective.
• Managing cancer pain with nonpharmacologic and complementary therapies.
• Molecular cloning and biochemical characterization of sialidases from zebrafish (Danio rerio).
• New polyphenols active on beta-amyloid aggregation.
• Peer enhancement of a brief motivational intervention with mandated college students.
• Production of beta-galactosidase by Trichoderma reesei FTKO-39 in wheat bran: partial purification of two isozymes.
• Stepped care for mandated college students: a pilot study.
• Superior vena cava syndrome due to chronic granulomatous mediastinitis.
• Use of oral cholera vaccine in complex emergencies: what next? Summary report of an expert meeting and recommendations of WHO.
• [Blood protein fractionation by mean of salting out; protein solubility curves.]
• [Hyperthyroidism]
• [Iron and pernicious anemia.]
• [Leonardo da Vinci, a self-taught anatomist.]
• [Leonardo's ideas concerning vision.]
• [Multiple ripetitive mammary fibroadenomas. Case report]
• [Obesity; considerations on its pathogenesis and therapy.]
• [Pregnancy and delivery after pulmonary excision.]
• [Primary prevention of intermittent claudication and frequently associated arteriopathies]
• [Sequels of tuberculous meningitis.]
• [THE PROBLEM OF COUGHING DURING THE POSTOPERATIVE PERIOD. THERAPEUTIC CONTRIBUTION.]
• [THE RENAL CONTROL OF HEMOPOIESIS: ERYTHROPOIETIN.]