Fusidic acid-resistant EF-G perturbs the accumulation of ppGpp.

Fusidic acid-resistant EF-G perturbs the accumulation of ppGpp. Research Abstract Details 

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Fusidic acid-resistant EF-G perturbs the accumulation of ppGpp. Abstract Text:

M Macvanin,U Johanson,M Ehrenberg,D Hughes,

Reductions in growth rate caused by fusidic acid-resistant EF-G mutants in Salmonella typhimurium correlate strongly with increased mean cell size. This is unusual because growth rate and cell size normally correlate positively. The global transcription regulator molecule ppGpp has a role in co-ordinating growth rate and division, and its basal level normally correlates inversely with cell size at division. We show that fusidic acid-resistant EF-G mutants have perturbed ppGpp basal levels during steady-state growth and perturbed induced levels during starvation. One mutation, fusA1, associated with the slowest growth rate and largest cell size, causes a reduction in the basal level of ppGpp to one-third of that found in the wild-type strain. Other fusA mutants with intermediate or wild-type growth rates and cell sizes have either normal or increased basal levels of ppGpp. There is an inverse relationship between the basal level of ppGpp in vivo and the degree to which translation dependent on mutant EF-G is inhibited by ppGpp in vitro. This enhanced interaction between mutant EF-G and ppGpp correlates with an increased KM for GTP. Our results suggest that mutant EF-G modulates the production of ppGpp by the RelA (PSI) pathway. In conclusion, fusidic acid-resistant EF-G mutations alter the level of ppGpp and break the normal relationship between growth rate and cell size at division. It would not be surprising if other phenotypes associated with these mutants, such as loss of virulence, were also related to perturbations in ppGpp levels effected through altered transcription patterns.

Fusidic acid-resistant EF-G perturbs the accumulation of ppGpp. Publishing Authors By Initials

M Macvanin,U Johanson,M Ehrenberg,D Hughes,

For similar bacteria: gram-negative bacteria: gram-negative facultatively anaerobic rods: enterobacteriaceae: salmonella: salmonella enterica: salmonella typhimurium research abstracts see: bacteria: gram-negative bacteria: gram-negative facultatively anaerobic rods: enterobacteriaceae: salmonella: salmonella enterica: salmonella typhimurium research

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Fusidic acid-resistant EF-G perturbs the accumulation of ppGpp. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Molecular microbiology

VOLUME: 37

Page Numbers: 98-107

Journal Abbreviation: Mol. Microbiol.

ISSN: 0950-382X

DAY: 19

MONTH: Jul

YEAR: 2000

Fusidic acid-resistant EF-G perturbs the accumulation of ppGpp. Information

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LANGUAGE: eng

NlmUniqueID: 8712028

Fusidic acid-resistant EF-G perturbs the accumulation of ppGpp. Keywords Mesh Terms:

KEYWORDS: Salmonella typhimurium

MESH TERMS: metabolism

Chemical & Substance for Abstract: Fusidic acid-resistant EF-G perturbs the accumulation of ppGpp. Information

Substance Name: Fusidic Acid

Registry Number: 6990-06-3

Grant and Affiliation Information for Fusidic acid-resistant EF-G perturbs the accumulation of ppGpp.

AFFILIATION: Department of Cell and Molecular Biology, Box 596, The Biomedical Center, Uppsala University, S-751 24 Uppsala, Sweden.

Country: ENGLAND

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MEDLINETA: Mol Microbiol

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