Alkalophilic Bacillus No. 8-1 and No. 10A-2, isolated on alkaline media containing NaHCO3 and K2CO3, respectively, required Na+ specifically for the uptake of alpha-aminoisobutyric acid (AIB) into the cells. The K+ added to the uptake medium inhibited the accumulation of AIB in the cells of Bacillus No, 8-1, acting as a competitive inhibitor of the system. Sodium ion prevented the release of AIB from pre-loaded cells. Both potassium and lithium ions had the effect of retaining the AIB pool. The Vmax value for transport increased about five-fold when the pH value of medium was raised from 7 to 9, whereas the Km value decreased with the increase of concentration of sodium ion in the medium of pH 7 or 9. Sodium ion gradient across the membrane caused transient uptake by AIB by membrane vesicles of Bacillus No. 8-1.
Further properties of sodium ion-stimulated alpha-[1-14C]aminoisobutyric acid uptake in alkalophilic Bacillus species. Publishing Authors By Initials
Further properties of sodium ion-stimulated alpha-[1-14C]aminoisobutyric acid uptake in alkalophilic Bacillus species. Journal Published:
PUBLICATION TYPE: Journal Article
Journal: Journal of biochemistry
VOLUME: 87
Page Numbers: 1279-84
Journal Abbreviation: J. Biochem.
ISSN: 0021-924X
DAY: 19
MONTH: May
YEAR: 1980
Further properties of sodium ion-stimulated alpha-[1-14C]aminoisobutyric acid uptake in alkalophilic Bacillus species. Information
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LANGUAGE: eng
NlmUniqueID: 376600
Further properties of sodium ion-stimulated alpha-[1-14C]aminoisobutyric acid uptake in alkalophilic Bacillus species. Keywords Mesh Terms:
KEYWORDS: Sodium
MESH TERMS: pharmacology
Chemical & Substance for Abstract: Further properties of sodium ion-stimulated alpha-[1-14C]aminoisobutyric acid uptake in alkalophilic Bacillus species. Information
Substance Name: Sodium
Registry Number: 7440-23-5
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MEDLINETA: J Biochem
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