Functional studies of intimin in vivo and ex vivo: implications for host specificity and tissue tropism.

Functional studies of intimin in vivo and ex vivo: implications for host specificity and tissue tropism. Research Abstract Details 

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Functional studies of intimin in vivo and ex vivo: implications for host specificity and tissue tropism. Abstract Text:

Rosanna Mundy,Stephanie ,Francis Girard,John M Fairbrother,Alan D Phillips,Gad Frankel,

Intimin is an outer-membrane adhesin that is essential for colonization of the host gastrointestinal tract by attaching and effacing pathogens including enteropathogenic Escherichia coli (EPEC), enterohaemorrhagic E. coli (EHEC) and Citrobacter rodentium (CR). The N-terminus of intimin from the different strains is highly conserved while the C-terminus, which harnesses the active receptor-binding site, shows sequence and antigenic polymorphism. This diversity was used to define a number of distinct intimin types, the most common of which are alpha, beta and gamma. Intimin binds the type III secretion system effector protein Tir. However, a large body of evidence suggests that intimin also binds a host-cell-encoded receptor(s) (Hir), and interaction of different intimin types with Hir contributes to tissue and host specificity. The aims of this study were to compare the activity of the major intimin types (alpha, beta and gamma) in vivo and ex vivo, using the CR mouse model and in vitro organ culture (IVOC), and to determine their exchangeability. The results confirm that intimin gamma is not functional in the CR mouse model. In the pig, intimin beta can substitute for EPEC intimin alpha but when placed in an EHEC O157 : H7 background it does not produce an intimin alpha-like tropism, although some adhesion to the small and large intestine was observed. In contrast, in human IVOC, intimin beta in an EHEC background produces small intestinal colonization in a similar manner to intimin alpha.

Functional studies of intimin in vivo and ex vivo: implications for host specificity and tissue tropism. Publishing Authors By Initials

R Mundy,S ,F Girard,JM Fairbrother,AD Phillips,G Frankel,

For similar biological phenomena, cell phenomena, and immunity: biological phenomena: microbiologic phenomena: virulence research abstracts see: biological phenomena, cell phenomena, and immunity: biological phenomena: microbiologic phenomena: virulence research

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Functional studies of intimin in vivo and ex vivo: implications for host specificity and tissue tropism. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Microbiology (Reading, England)

VOLUME: 153

Page Numbers: 959-67

Journal Abbreviation: Microbiology (Reading, Engl.)

ISSN: 1350-0872

DAY: 28

MONTH: Apr

YEAR: 2007

Functional studies of intimin in vivo and ex vivo: implications for host specificity and tissue tropism. Information

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LANGUAGE: eng

NlmUniqueID: 9430468

Functional studies of intimin in vivo and ex vivo: implications for host specificity and tissue tropism. Keywords Mesh Terms:

KEYWORDS: Virulence

MESH TERMS: microbiology

Chemical & Substance for Abstract: Functional studies of intimin in vivo and ex vivo: implications for host specificity and tissue tropism. Information

Substance Name: eaeA protein, E coli

Registry Number: 147094-99-3

Grant and Affiliation Information for Functional studies of intimin in vivo and ex vivo: implications for host specificity and tissue tropism.

AFFILIATION: Division of Cell and Molecular Biology, Imperial College London, London, UK.

Country: England

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MEDLINETA: Microbiology

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