Functional role of CCCTC binding factor in insulin-stimulated cell proliferation.

Functional role of CCCTC binding factor in insulin-stimulated cell proliferation. Research Abstract Details 

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Functional role of CCCTC binding factor in insulin-stimulated cell proliferation. Abstract Text:

J Gao,T Li,L Lu,J Gao,T Li,L Lu,

OBJECTIVES: CCCTC binding factor (CTCF) is a nuclear protein containing an 11-zinc-finger DNA-binding domain. CTCF plays important roles in the regulation of epigenetics and gene transcription. As a multifunctional protein, CTCF is also involved in the regulation of cell proliferation and of apoptosis. However, mechanisms underlining the regulatory function of CTCF in mediating growth factor- and cytokine-stimulated cell fate are largely unknown. MATERIALS AND METHODS: The effect of CTCF on insulin-induced ML-1 cell proliferation was investigated by studying insulin-stimulated extracellular signal-regulated kinase (Erk) and Akt signalling pathways, and the alterations of CTCF activity in these cells. RESULTS: The present study demonstrates that insulin-induced human haematopoietic myeloblastic ML-1 cell proliferation requires increased CTCF expression. Inhibition of Erk and Akt pathways with specific blockers or by dominantly negative expression of Erk and Akt mutants markedly suppressed expression of CTCF and resulted in retardation of cell proliferation. Furthermore, insulin-induced ML-1 cell proliferation was significantly enhanced by overexpression of cDNA encoding full-length CTCF. In contrast, ML-1 cell proliferation was inhibited by knocking down CTCF mRNA using specific small interference RNA. CONCLUSIONS: Our results indicate that CTCF is indeed a protein with multifunctional activity that plays a significant role in modulating signalling pathways to mediate insulin-induced ML-1 cell proliferation.

Functional role of CCCTC binding factor in insulin-stimulated cell proliferation. Publishing Authors By Initials

J Gao,T Li,L Lu,J Gao,T Li,L Lu,

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Functional role of CCCTC binding factor in insulin-stimulated cell proliferation. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Cell proliferation

VOLUME: 40

Page Numbers: 795-808

Journal Abbreviation: Cell Prolif.

ISSN: 1365-2184

DAY: 20

MONTH: Dec

YEAR: 2007

Functional role of CCCTC binding factor in insulin-stimulated cell proliferation. Information

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LANGUAGE: eng

NlmUniqueID: 9105195

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Grant and Affiliation Information for Functional role of CCCTC binding factor in insulin-stimulated cell proliferation.

AFFILIATION: Department of Medicine, David Geffen School of Medicine, University of California Los Angeles, Torrance, CA 90502, USA.

Country: England

AGENCY: United States NEI

GRANT: EY15282

ACRONYM: EY

MEDLINETA: Cell Prolif

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