Functional polymorphism of the Anpep gene increases promoter activity in the Dahl salt-resistant rat.

Functional polymorphism of the Anpep gene increases promoter activity in the Dahl salt-resistant rat. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Functional polymorphism of the Anpep gene increases promoter activity in the Dahl salt-resistant rat. Abstract Text:

Kumar Kotlo,Douglas E Hughes,Victoria L M Herrera,Nelson Ruiz-Opazo,Robert H Costa,R Brooks Robey,Robert S Danziger,

We have reported that aminopeptidase N/CD13, which metabolizes angiotensin III to angiotensin IV, exhibits greater renal tubular expression in the Dahl salt-resistant (SR/Jr) rat than its salt-sensitive (SS/Jr) counterpart. In this work, aminopeptidase N (Anpep) genes from SS/Jr and SR/Jr strains were compared. The coding regions contained only silent single nucleotide polymorphisms between strains. The 5' flanking regions also contained multiple single nucleotide polymorphisms, which were analyzed by electrophoretic mobility-shift assay using renal epithelial cell (HK-2) nuclear extracts and oligonucleotides corresponding with single nucleotide polymorphism-containing regions. A unique single nucleotide polymorphism 4 nucleotides upstream of a putative CCAAT/enhancer binding protein motif (nucleotides -2256 to -2267) in the 5' flanking region of the SR/Jr Anpep gene was associated with DNA-protein complex formation, whereas the corresponding sequences in SS rats were not. A chimeric reporter gene containing approximately 4.4 Kb of Anpep 5' flank from the Dahl SR/Jr rat exhibited 2.5- to 3-fold greater expression in HK-2 cells than the corresponding construct derived from the SS strain (P<0.05). Replacing the CCAAT/enhancer binding protein cis-acting element from the SS rat with that from the SR strain increased reporter gene expression by 2.5-fold (P<0.05) and abolished this difference. CCAAT/enhancer binding protein association was confirmed by chromatin immunoprecipitation and correlated with expression, suggesting selection for a functional CCAAT/enhancer binding protein polymorphism in the 5' flank of Anpep in the Dahl SR/Jr rat. These results highlight a possible association of the Anpep gene with hypertension in Dahl rat and raise the prospect that increased Anpep may play a mechanistic role in adaptation to high salt.

Functional polymorphism of the Anpep gene increases promoter activity in the Dahl salt-resistant rat. Publishing Authors By Initials

K Kotlo,DE Hughes,VL Herrera,N Ruiz-Opazo,RH Costa,RB Robey,RS Danziger,

For similar animals: animal population groups: animals, inbred strains: rats, inbred strains: rats, inbred lew research abstracts see: animals: animal population groups: animals, inbred strains: rats, inbred strains: rats, inbred lew research

PUBMED ID PMID:

MEDLINE DATE:

Functional polymorphism of the Anpep gene increases promoter activity in the Dahl salt-resistant rat. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Hypertension

VOLUME: 49

Page Numbers: 467-72

Journal Abbreviation: Hypertension

ISSN: 1524-4563

DAY: 22

MONTH: 01

YEAR: 2007

Functional polymorphism of the Anpep gene increases promoter activity in the Dahl salt-resistant rat. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7906255

Functional polymorphism of the Anpep gene increases promoter activity in the Dahl salt-resistant rat. Keywords Mesh Terms:

KEYWORDS: Rats, Inbred Lew

MESH TERMS: genetics

Chemical & Substance for Abstract: Functional polymorphism of the Anpep gene increases promoter activity in the Dahl salt-resistant rat. Information

Substance Name: Antigens, CD13

Registry Number: EC 3.4.11.2

Grant and Affiliation Information for Functional polymorphism of the Anpep gene increases promoter activity in the Dahl salt-resistant rat.

AFFILIATION: Department of Medicine, University of Illinois at Chicago, 840 S Wood St, Chicago, IL 60612. RDanziger@aol.com.

Country: United States

AGENCY: United States NIDDK

GRANT: DK54687-06

ACRONYM: DK

MEDLINETA: Hypertension

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Functional polymorphism of the Anpep gene increases promoter activity in the Dahl salt-resistant rat Related Publications

• Accidental poisoning by vinyl chloride: report of two cases.
• Acute inhalation toxicity of vinyl chloride to laboratory animals.
• Aminopeptidase N in arterial hypertension.
• Aminopeptidase N reduces basolateral Na+ -K+ -ATPase in proximal tubule cells.
• Computed tomography in cranial tuberculosis.
• Entropy, mental disease and death.
• Functional polymorphism of the Anpep gene increases promoter activity in the Dahl salt-resistant rat.
• Importance of low-grade albuminuria.
• Instrumentality--terminality values in relation to age.
• Is anaesthetist dentist's agent?
• On cyberslacking: workplace status and personal internet use at work.
• Radiographic features of olfactory neuroblastoma.
• Renal Phosphodiesterase 4B Is Activated in the Dahl Salt-Sensitive Rat.
• Safer dental anaesthesia.
• The aging of man and medicine.
• The bone-renal axis in early chronic kidney disease: an emerging paradigm.
• The determination of prognosis.
• Twin pregnancy with acute hydramnios treated by paracentesis uteri.
• Vitamin K-dependent proteins, warfarin, and vascular calcification.