Functional genomics in Trypanosoma brucei identifies evolutionarily conserved components of motile flagella.

Functional genomics in Trypanosoma brucei identifies evolutionarily conserved components of motile flagella. Research Abstract Details 

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Functional genomics in Trypanosoma brucei identifies evolutionarily conserved components of motile flagella. Abstract Text:

Desiree M Baron,Katherine S Ralston,Zakayi P Kabututu,Kent L Hill,

Cilia and flagella are highly conserved, complex organelles involved in a variety of important functions. Flagella are required for motility of several human pathogens and ciliary defects lead to a variety of fatal and debilitating human diseases. Many of the major structural components of cilia and flagella are known, but little is known about regulation of flagellar beat. Trypanosoma brucei, the causative agent of African sleeping sickness, provides an excellent model for studying flagellar motility. We have used comparative genomics to identify a core group of 50 genes unique to organisms with motile flagella. These genes, referred to as T. brucei components of motile flagella (TbCMF) include 30 novel genes, and human homologues of many of the TbCMF genes map to loci associated with human ciliary diseases. To characterize TbCMF protein function we used RNA interference to target 41 TbCMF genes. Sedimentation assays and direct observation demonstrated clear motility defects in a majority of these knockdown mutants. Epitope tagging, fluorescence localization and biochemical fractionation demonstrated flagellar localization for several TbCMF proteins. Finally, ultrastructural analysis identified a family of novel TbCMF proteins that function to maintain connections between outer doublet microtubules, suggesting that they are the first identified components of nexin links. Overall, our results provide insights into the workings of the eukaryotic flagellum, identify several novel human disease gene candidates, reveal unique aspects of the trypanosome flagellum and underscore the value of T. brucei as an experimental system for studying flagellar biology.

Functional genomics in Trypanosoma brucei identifies evolutionarily conserved components of motile flagella. Publishing Authors By Initials

DM Baron,KS Ralston,ZP Kabututu,KL Hill,

For similar animals: invertebrates: protozoa: sarcomastigophora: mastigophora: zoomastigophora: kinetoplastida: trypanosomatina: trypanosoma: trypanosoma brucei brucei research abstracts see: animals: invertebrates: protozoa: sarcomastigophora: mastigophora: zoomastigophora: kinetoplastida: trypanosomatina: trypanosoma: trypanosoma brucei brucei research

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Functional genomics in Trypanosoma brucei identifies evolutionarily conserved components of motile flagella. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of cell science

VOLUME: 120

Page Numbers: 478-91

Journal Abbreviation: J. Cell. Sci.

ISSN: 0021-9533

DAY: 16

MONTH: 01

YEAR: 2007

Functional genomics in Trypanosoma brucei identifies evolutionarily conserved components of motile flagella. Information

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LANGUAGE: eng

NlmUniqueID: 52457

Functional genomics in Trypanosoma brucei identifies evolutionarily conserved components of motile flagella. Keywords Mesh Terms:

KEYWORDS: Trypanosoma brucei brucei

MESH TERMS: physiology

Chemical & Substance for Abstract: Functional genomics in Trypanosoma brucei identifies evolutionarily conserved components of motile flagella. Information

Substance Name: Green Fluorescent Proteins

Registry Number: 147336-22-9

Grant and Affiliation Information for Functional genomics in Trypanosoma brucei identifies evolutionarily conserved components of motile flagella.

AFFILIATION: Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, CA 90095, USA.

Country: England

AGENCY: United States NIAID

GRANT: R01AI52348

ACRONYM: AI

MEDLINETA: J Cell Sci

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