Functional expression of m3, a muscarinic acetylcholine receptor subtype, in taste bud cells of mouse fungiform papillae.

Functional expression of m3, a muscarinic acetylcholine receptor subtype, in taste bud cells of mouse fungiform papillae. Research Abstract Details 

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Functional expression of m3, a muscarinic acetylcholine receptor subtype, in taste bud cells of mouse fungiform papillae. Abstract Text:

Kohgaku Eguchi,Yoshitaka Ohtubo,Kiyonori Yoshii,

Taste bud cells (TBCs) express various neurotransmitter receptors assumed to facilitate or modify taste information processing within taste buds. We investigated the functional expression of muscarinic acetylcholine receptor (mAChR) subtypes, M1-M5, in mouse fungiform TBCs. ACh applied to the basolateral membrane of TBCs elevates the intracellular Ca(2+) level in a concentration-dependent manner with the 50% effective concentration (EC(50)) of 0.6 muM. The Ca(2+) responses occur in the absence of extracellular Ca(2+) and are inhibited by atropine, a selective antagonist against mAChRs. The order of 50% inhibitory concentration (IC(50)) examined with a series of antagonists selective to mAChR subtypes shows the expression of M3 on TBCs. Perforated whole-cell voltage clamp studies show that 1 muM ACh blocks an outwardly rectifying current and that 100 nM atropine reverses the block. Reverse transcriptase-mediated polymerase chain reaction studies suggest the expression of M3 but not the other mAChR subtypes. Immunohistochemical studies show that phospholipase Cbeta-immunoreactive TBCs and synaptosome-associated protein of 25 kDa-immunoreactive nerve endings are immunoreactive to a transporter that packs ACh molecules into synaptic vesicles (vesicular acetylcholine transporter). These results show that M3 occurs on a few fungiform TBCs and suggest that a few nerve endings, and probably a few TBCs, release ACh by exocytosis. The role of ACh in taste responses is discussed.

Functional expression of m3, a muscarinic acetylcholine receptor subtype, in taste bud cells of mouse fungiform papillae. Publishing Authors By Initials

K Eguchi,Y Ohtubo,K Yoshii,

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Functional expression of m3, a muscarinic acetylcholine receptor subtype, in taste bud cells of mouse fungiform papillae. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Chemical senses

VOLUME: 33

Page Numbers: 47-55

Journal Abbreviation: Chem. Senses

ISSN: 0379-864X

DAY: 13

MONTH: 09

YEAR: 2007

Functional expression of m3, a muscarinic acetylcholine receptor subtype, in taste bud cells of mouse fungiform papillae. Information

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LANGUAGE: eng

NlmUniqueID: 8217190

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AFFILIATION: Hibikino 2-4, Graduate School of Life Science and Systems Engineering, Kyushu Institute of Technology, Kitakyushu 808-0196, Japan. yoshii@brain.kyutech.ac.jp.

Country: England

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MEDLINETA: Chem Senses

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