Functional deficiency in IL-7 caused by an N-ethyl-N-nitrosourea-induced point mutation.

Functional deficiency in IL-7 caused by an N-ethyl-N-nitrosourea-induced point mutation. Research Abstract Details 

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Functional deficiency in IL-7 caused by an N-ethyl-N-nitrosourea-induced point mutation. Abstract Text:

Jianxun Feng,Hongsheng Wang,Herbert C Morse,

N-ethyl-N-nitrosourea (ENU)-induced mutagenesis provides a powerful approach for identifying genes involved in immune regulation and diseases. Here we describe a new mutant strain, HLB368, with hereditary leukopenia. At necropsy, the mutant mice had very small thymuses and spleens. All but the inguinal nodes were absent and there were no Peyer's patches. By flow cytometry, the ratios of T-cell subsets were normal, but B-cell development was blocked at the pre-pro-B-cell stage. The development of B1 and marginal zone B cells was relatively normal. The mutation was mapped to chromosome 3 between D3Mit221 and D3Mit224, a region that contains the Il7 gene. cDNA and genomic DNA sequences of Il7 revealed a T-to-C missense transition resulting in a change of Leu to Pro within the leader peptide that would be predicted to inhibit secretion. In keeping with this concept, we found that in vitro treatment of B-cell progenitors from mutant mice with IL-7 induced them to differentiate into pre-BII cells. Phenotypic comparisons of HLB368 with genetically targeted Il7 null mice showed many similarities along with a few differences, indicating that this ENU-induced mutant carries a novel allele. This new strain thus provides a new model for studying the functions of IL-7 on a pure C57BL/6 background.

Functional deficiency in IL-7 caused by an N-ethyl-N-nitrosourea-induced point mutation. Publishing Authors By Initials

J Feng,H Wang,HC Morse,

For similar tissues: lymphoid tissue: thymus gland research abstracts see: tissues: lymphoid tissue: thymus gland research

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Functional deficiency in IL-7 caused by an N-ethyl-N-nitrosourea-induced point mutation. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Intr

Journal: Genetics

VOLUME: 175

Page Numbers: 545-51

Journal Abbreviation: Genetics

ISSN: 0016-6731

DAY: 18

MONTH: 12

YEAR: 2006

Functional deficiency in IL-7 caused by an N-ethyl-N-nitrosourea-induced point mutation. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 374636

Functional deficiency in IL-7 caused by an N-ethyl-N-nitrosourea-induced point mutation. Keywords Mesh Terms:

KEYWORDS: Thymus Gland

MESH TERMS: drug effects

Chemical & Substance for Abstract: Functional deficiency in IL-7 caused by an N-ethyl-N-nitrosourea-induced point mutation. Information

Substance Name: Ethylnitrosourea

Registry Number: 759-73-9

Grant and Affiliation Information for Functional deficiency in IL-7 caused by an N-ethyl-N-nitrosourea-induced point mutation.

AFFILIATION: Laboratory of Immunopathology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, Maryland 20852, USA.

Country: United States

AGENCY: United States NHLBI

GRANT: HL66611

ACRONYM: HL

MEDLINETA: Genetics

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