Functional comparison of T cells recognizing cytomegalovirus pp65 and intermediate-early antigen polypeptides in hematopoietic stem-cell transplant and solid organ transplant recipients.

Functional comparison of T cells recognizing cytomegalovirus pp65 and intermediate-early antigen polypeptides in hematopoietic stem-cell transplant and solid organ transplant recipients. Research Abstract Details 

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Functional comparison of T cells recognizing cytomegalovirus pp65 and intermediate-early antigen polypeptides in hematopoietic stem-cell transplant and solid organ transplant recipients. Abstract Text:

Simon F Lacey,Corinna La Rosa,Wendy Zhou,Madeva C Sharma,Joy Martinez,Aparna Krishnan,Ghislaine Gallez-Hawkins,Lia Thao,Jeff Longmate,Ricardo Spielberger,Stephen J Forman,Ajit Limaye,John A Zaia,Don J Diamond,

The functional status of cytotoxic T lymphocyte (CTL) populations recognizing cytomegalovirus intermediate-early antigen (IE1) and pp65 polypeptides was investigated in peripheral blood mononuclear cells from hematopoietic stem-cell transplant (HSCT) and solid organ transplant recipients. Combined flow-based CD107a/b degranulation/mobilization and intracellular cytokine (ICC) assays using peptide libraries as antigens indicated that a significantly higher proportion of pp65-specific CTLs were in a more mature functional state, compared with IE1-specific CTLs. Degranulation/multiple cytokine ICC assays also indicated that a significantly higher proportion of pp65-specific than IE1-specific CTLs secreted both interferon- gamma and tumor necrosis factor- alpha and possessed greater cytotoxic potential. These results support our earlier findings of functional differences between CTLs recognizing individual epitopes within the IE1 and pp65 antigens in healthy donors and HSCT recipients and extend them to a broader array of human leukocyte antigen-restricted responses to those antigens. We also provide evidence of a relationship between cytotoxic function and the ability of cytomegalovirus-specific CTLs to secrete multiple cytokines.

Functional comparison of T cells recognizing cytomegalovirus pp65 and intermediate-early antigen polypeptides in hematopoietic stem-cell transplant and solid organ transplant recipients. Publishing Authors By Initials

SF Lacey,C La Rosa,W Zhou,MC Sharma,J Martinez,A Krishnan,G Gallez-Hawkins,L Thao,J Longmate,R Spielberger,SJ Forman,A Limaye,JA Zaia,DJ Diamond,

For similar proteins: viral proteins research abstracts see: proteins: viral proteins research

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Functional comparison of T cells recognizing cytomegalovirus pp65 and intermediate-early antigen polypeptides in hematopoietic stem-cell transplant and solid organ transplant recipients. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: The Journal of infectious diseases

VOLUME: 194

Page Numbers: 1410-21

Journal Abbreviation: J. Infect. Dis.

ISSN: 0022-1899

DAY: 11

MONTH: 10

YEAR: 2006

Functional comparison of T cells recognizing cytomegalovirus pp65 and intermediate-early antigen polypeptides in hematopoietic stem-cell transplant and solid organ transplant recipients. Information

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LANGUAGE: eng

NlmUniqueID: 413675

Functional comparison of T cells recognizing cytomegalovirus pp65 and intermediate-early antigen polypeptides in hematopoietic stem-cell transplant and solid organ transplant recipients. Keywords Mesh Terms:

KEYWORDS: Viral Proteins

MESH TERMS: immunology

Chemical & Substance for Abstract: Functional comparison of T cells recognizing cytomegalovirus pp65 and intermediate-early antigen polypeptides in hematopoietic stem-cell transplant and solid organ transplant recipients. Information

Substance Name: cytomegalovirus matrix protein 65kDa

Registry Number: 0

Grant and Affiliation Information for Functional comparison of T cells recognizing cytomegalovirus pp65 and intermediate-early antigen polypeptides in hematopoietic stem-cell transplant and solid organ transplant recipients.

AFFILIATION: Laboratory of Vaccine Research, Division of Information Sciences, Beckman Research Institute of the City of Hope, City of Hope Comprehensive Cancer Center, Duarte, CA 91010-3000, USA. slacey@coh.org

Country: United States

AGENCY: United States NCI

GRANT: R01-CA77544

ACRONYM: CA

MEDLINETA: J Infect Dis

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