Functional BMP receptor in endocardial cells is required in atrioventricular cushion mesenchymal cell formation in chick.

Functional BMP receptor in endocardial cells is required in atrioventricular cushion mesenchymal cell formation in chick. Research Abstract Details 

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Functional BMP receptor in endocardial cells is required in atrioventricular cushion mesenchymal cell formation in chick. Abstract Text:

Hiroto Okagawa,Roger R Markwald,Yukiko Sugi,

Transformation of atrioventricular (AV) canal endocardium into invasive mesenchyme correlates spatially and temporally with the expression of bone morphogenetic protein (BMP)-2 in the AV myocardium. We revealed the presence of mRNA of Type I BMP receptors, BMPR-1A (ALK3), BMPR-1B (ALK6) and ALK2 in chick AV endocardium at stage-14(-), the onset of epithelial to mesenchymal transformation (EMT), by RT-PCR and localized BMPR-1B mRNA in the endocardium by in situ hybridization. To circumvent the functional redundancies among the Type I BMP receptors, we applied dominant-negative (dn) BMPR-1B-viruses to chick AV explants and whole-chick embryo cultures to specifically block BMP signaling in AV endocardium during EMT. dnBMPR-1B-virus infection of AV endocardial cells abolished BMP-2-supported AV endocardial EMT. Conversely, caBMPR-1B-virus infection promoted AV endocardial EMT in the absence of AV myocardium. Moreover, dnBMPR-1B-virus treatments significantly reduced myocardially supported EMT in AV endocardial-myocardial co-culture. AV cushion mesenchymal cell markers, alpha-smooth muscle actin (SMA), and TGFbeta3 in the endocardial cells were promoted by caBMPR-1B and reduced by dnBMPR-1B infection. Microinjection of the virus into the cardiac jelly in the AV canal at stage-13 in vivo (ovo) revealed that the dnBMPR-1B-virus-infected cells remained in the endocardial epithelium, whereas caBMPR-1B-infected cells invaded deep into the cushions. These results provide evidence that BMP signaling through the AV endocardium is required for the EMT and the activation of the BMP receptor in the endocardium can promote AV EMT in the chick.

Functional BMP receptor in endocardial cells is required in atrioventricular cushion mesenchymal cell formation in chick. Publishing Authors By Initials

H Okagawa,RR Markwald,Y Sugi,

For similar peptides: intercellular signaling peptides and proteins: cytokines: transforming growth factor beta: transforming growth factor beta3 research abstracts see: peptides: intercellular signaling peptides and proteins: cytokines: transforming growth factor beta: transforming growth factor beta3 research

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Functional BMP receptor in endocardial cells is required in atrioventricular cushion mesenchymal cell formation in chick. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Developmental biology

VOLUME: 306

Page Numbers: 179-92

Journal Abbreviation: Dev. Biol.

ISSN: 0012-1606

DAY: 16

MONTH: 03

YEAR: 2007

Functional BMP receptor in endocardial cells is required in atrioventricular cushion mesenchymal cell formation in chick. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 372762

Functional BMP receptor in endocardial cells is required in atrioventricular cushion mesenchymal cell formation in chick. Keywords Mesh Terms:

KEYWORDS: Transforming Growth Factor beta3

MESH TERMS: metabolism

Chemical & Substance for Abstract: Functional BMP receptor in endocardial cells is required in atrioventricular cushion mesenchymal cell formation in chick. Information

Substance Name: Bone Morphogenetic Protein Receptors, Ty

Registry Number: EC 2.7.1.37

Grant and Affiliation Information for Functional BMP receptor in endocardial cells is required in atrioventricular cushion mesenchymal cell formation in chick.

AFFILIATION: Department of Cell Biology and Anatomy and Cardiovascular Developmental Biology Center, Medical University of South Carolina, 171 Ashley Avenue, Charleston, SC 29425, USA. jefata@lbl.gov <jefata@lbl.gov>

Country: United States

AGENCY: United States NICHD

GRANT: N01-HD-6-2915

ACRONYM: HD

MEDLINETA: Dev Biol

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