Functional and Structural Characterization of EnvZ, an Osmosensing Histidine Kinase of E. coli.

Functional and Structural Characterization of EnvZ, an Osmosensing Histidine Kinase of E. coli. Research Abstract Details 

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Functional and Structural Characterization of EnvZ, an Osmosensing Histidine Kinase of E. coli. Abstract Text:

Takeshi Yoshida,Sangita Phadtare,Masayori Inouye,Takeshi Yoshida,Sangita Phadtare,Masayori Inouye,Takeshi Yoshida,Sangita Phadtare,Masayori Inouye,

EnvZ is an osmosensing histidine kinase located in the inner membrane, and one of the most extensively studied Escherichia coli histidine kinases. Because of its structural complexity, functional and structural studies have been quite challenging. It is a multidomain transmembrane protein consisting of 450 amino acid residues. In addition, it must form a dimer to function as a histidine kinase like all the other histidine kinases. EnvZ consists of the 115-residue periplasmic domain, two transmembrane domains (TM1 and TM2), and the cytoplasmic domain consisting of the 43-residue linker (HAMP) domain and the 228-residue kinase domain. It has been shown that the kinase domain of EnvZ, responsible for its enzymatic activities, contains all of the conserved regions of histidine kinases such as H, F, N, G1, G2, and G3 boxes. Therefore, the 271-residue cytoplasmic domain of EnvZ (termed EnvZc) has been used as a model system to establish fundamental characteristics of histidine kinases. The DNA fragment encoding EnvZc was cloned in pET vector and EnvZc was expressed and purified. It is highly soluble and retains all the enzymatic activities of EnvZ. We demonstrated that it consists of two functional domains, domain A and domain B. NMR spectroscopic studies of these two domains revealed, for the first time, the structure of a histidine kinase. Domain A is responsible for dimerization of EnvZc forming a four-helical bundle containing two alpha-helical hairpin structures, while domain B is a monomer and has an ATP-binding pocket formed by regions conserved among the histidine kinases. In this chapter, we describe functional and structural studies of EnvZc, which can be applied to characterize other histidine kinases.

Functional and Structural Characterization of EnvZ, an Osmosensing Histidine Kinase of E. coli. Publishing Authors By Initials

T Yoshida,S Phadtare,M Inouye,T Yoshida,S Phadtare,M Inouye,T Yoshida,S Phadtare,M Inouye,

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Functional and Structural Characterization of EnvZ, an Osmosensing Histidine Kinase of E. coli. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Methods in enzymology

VOLUME: 423

Page Numbers: 184-202

Journal Abbreviation: Meth. Enzymol.

ISSN: 0076-6879

DAY: 4

MONTH: 07

YEAR: 2007

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LANGUAGE: eng

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