Functional analysis of an individual IFT protein: IFT46 is required for transport of outer dynein arms into flagella.

Functional analysis of an individual IFT protein: IFT46 is required for transport of outer dynein arms into flagella. Research Abstract Details 

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Functional analysis of an individual IFT protein: IFT46 is required for transport of outer dynein arms into flagella. Abstract Text:

Yuqing Hou,Hongmin Qin,John A Follit,Gregory J Pazour,Joel L Rosenbaum,George B Witman,

Intraflagellar transport (IFT), which is the bidirectional movement of particles within flagella, is required for flagellar assembly. IFT particles are composed of approximately 16 proteins, which are organized into complexes A and B. We have cloned Chlamydomonas reinhardtii and mouse IFT46, and show that IFT46 is a highly conserved complex B protein in both organisms. A C. reinhardtii insertional mutant null for IFT46 has short, paralyzed flagella lacking dynein arms and with central pair defects. The mutant has greatly reduced levels of most complex B proteins, indicating that IFT46 is necessary for complex B stability. A partial suppressor mutation restores flagellar length to the ift46 mutant. IFT46 is still absent, but levels of the other IFT particle proteins are largely restored, indicating that complex B is stabilized in the suppressed strain. Axonemal ultrastructure is restored, except that the outer arms are still missing, although outer arm subunits are present in the cytoplasm. Thus, IFT46 is specifically required for transporting outer arms into the flagellum.

Functional analysis of an individual IFT protein: IFT46 is required for transport of outer dynein arms into flagella. Publishing Authors By Initials

Y Hou,H Qin,JA Follit,GJ Pazour,JL Rosenbaum,GB Witman,

For similar investigative techniques: genetic techniques: sequence alignment research abstracts see: investigative techniques: genetic techniques: sequence alignment research

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Functional analysis of an individual IFT protein: IFT46 is required for transport of outer dynein arms into flagella. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: The Journal of cell biology

VOLUME: 176

Page Numbers: 653-65

Journal Abbreviation: J. Cell Biol.

ISSN: 0021-9525

DAY: 20

MONTH: 02

YEAR: 2007

Functional analysis of an individual IFT protein: IFT46 is required for transport of outer dynein arms into flagella. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 375356

Functional analysis of an individual IFT protein: IFT46 is required for transport of outer dynein arms into flagella. Keywords Mesh Terms:

KEYWORDS: Sequence Alignment

MESH TERMS: physiology

Chemical & Substance for Abstract: Functional analysis of an individual IFT protein: IFT46 is required for transport of outer dynein arms into flagella. Information

Substance Name: Dynein ATPase

Registry Number: EC 3.6.4.2

Grant and Affiliation Information for Functional analysis of an individual IFT protein: IFT46 is required for transport of outer dynein arms into flagella.

AFFILIATION: Department of Cell Biology, University of Massachusetts Medical School, Worcester, MA 01655, USA.

Country: United States

AGENCY: United States NIDDK

GRANT: P30 DK32520

ACRONYM: DK

MEDLINETA: J Cell Biol

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