Fully Automated Four-Column Capillary LC-MS System for Maximizing Throughput in Proteomic Analyses.

Fully Automated Four-Column Capillary LC-MS System for Maximizing Throughput in Proteomic Analyses. Research Abstract Details 

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Fully Automated Four-Column Capillary LC-MS System for Maximizing Throughput in Proteomic Analyses. Abstract Text:

Eric A Livesay,Keqi Tang,Beverley K Taylor,Michael A Buschbach,Derek F Hopkins,Brian L Lamarche,Rui Zhao,Yufeng Shen,Daniel J Orton,Ronald J Moore,Ryan T Kelly,Harold R Udseth,Richard D Smith,Eric A Livesay,Keqi Tang,Beverley K Taylor,Michael A Buschbach,Derek F Hopkins,Brian L LaMarche,Rui Zhao,Yufeng Shen,Daniel J Orton,Ronald J Moore,Ryan T Kelly,Harold R Udseth,Richard D Smith,Eric A Livesay,Keqi Tang,Beverley K Taylor,Michael A Buschbach,Derek F Hopkins,Brian L LaMarche,Rui Zhao,Yufeng Shen,Daniel J Orton,Ronald J Moore,Ryan T Kelly,Harold R Udseth,Richard D Smith,

We describe a four-column, high-pressure capillary liquid chromatography (LC) system for robust, high-throughput liquid chromatography-mass spectrometry (LC-MS(/MS)) analyses. This system performs multiple LC separations in parallel, but staggers each of them such that the data-rich region of each separation is sampled sequentially. By allowing nearly continuous data acquisition, this design maximizes the use of the mass spectrometer. Each analytical column is connected to a corresponding ESI emitter in order to avoid the use of postcolumn switching and associated dead volume issues. Encoding translation stages are employed to sequentially position the emitters at the MS inlet. The high reproducibility of this system is demonstrated using consecutive analyses of global tryptic digest of the microbe Shewanella oneidensis.

Fully Automated Four-Column Capillary LC-MS System for Maximizing Throughput in Proteomic Analyses. Publishing Authors By Initials

EA Livesay,K Tang,BK Taylor,MA Buschbach,DF Hopkins,BL Lamarche,R Zhao,Y Shen,DJ Orton,RJ Moore,RT Kelly,HR Udseth,RD Smith,EA Livesay,K Tang,BK Taylor,MA Buschbach,DF Hopkins,BL LaMarche,R Zhao,Y Shen,DJ Orton,RJ Moore,RT Kelly,HR Udseth,RD Smith,EA Livesay,K Tang,BK Taylor,MA Buschbach,DF Hopkins,BL LaMarche,R Zhao,Y Shen,DJ Orton,RJ Moore,RT Kelly,HR Udseth,RD Smith,

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Fully Automated Four-Column Capillary LC-MS System for Maximizing Throughput in Proteomic Analyses. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Analytical chemistry

VOLUME: 80

Page Numbers: 294-302

Journal Abbreviation: Anal. Chem.

ISSN: 0003-2700

DAY: 29

MONTH: 11

YEAR: 2007

Fully Automated Four-Column Capillary LC-MS System for Maximizing Throughput in Proteomic Analyses. Information

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LANGUAGE: eng

NlmUniqueID: 370536

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Grant and Affiliation Information for Fully Automated Four-Column Capillary LC-MS System for Maximizing Throughput in Proteomic Analyses.

AFFILIATION: Biological Sciences Division, Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, Washington 99354.

Country: United States

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MEDLINETA: Anal Chem

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