Full-term development of bovine follicular oocytes matured in culture and fertilized in vitro.

Full-term development of bovine follicular oocytes matured in culture and fertilized in vitro. Research Abstract Details 

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Full-term development of bovine follicular oocytes matured in culture and fertilized in vitro. Abstract Text:

K Utsumi,H Kato,A Iritani,

Follicular oocytes were cultured for 28h in vitro and 91% of the oocytes reached the the second metaphase in culture. The penetration rate after insemination in vitro using frozen-thawed spermatozoa was 81%. After cultivation for 48h in vitro, 18% of the in vitro fertilized oocytes developed to the three- to four-cell stages and 21% of these developed to the six- to eight-cell stages. Following in vivo culture in the rabbit oviduct, 18% of six- to eight-cell and 5% of three- to four-cell embryos developed to the blastocyst stage. To confirm the full developmental competence, 11 blastocysts were transferred to recipient cows, and six (55%) cows became pregnant or delivered calves.

Full-term development of bovine follicular oocytes matured in culture and fertilized in vitro. Publishing Authors By Initials

K Utsumi,H Kato,A Iritani,

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Full-term development of bovine follicular oocytes matured in culture and fertilized in vitro. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Theriogenology

VOLUME: 35

Page Numbers: 695-703

Journal Abbreviation: Theriogenology

ISSN: 0093-691X

DAY: 26

MONTH: Apr

YEAR: 1991

Full-term development of bovine follicular oocytes matured in culture and fertilized in vitro. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Full-term development of bovine follicular oocytes matured in culture and fertilized in vitro.

AFFILIATION: Laboratory of Animal Reproduction, Faculty of Agriculture Kyoto University, Kyoto 606, Japan.

Country: United States

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MEDLINETA: Theriogenology

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